GST-fusion protein purification

Mike Landon mbzml at unicorn.nott.ac.uk
Thu Jan 25 09:06:58 EST 1996


Help !
Does anyone have the protocol for purifying GST-fusion proteins on
glutathione-Sepharose please ? What I need to know is the concentration of
free glutathione required to elute the fusion, and whether the glutathione
needs to be oxidised or reduced ..

	Many thanks    Rob



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