Cell Culture Question
Simon.Dawson at nott.ac.uk
Fri Jan 26 07:39:27 EST 1996
A question about cell cuture techniques. Well, one technique in particular
I have some Swiss 3T3 cells and I would like to make them go quiescent (i.e.
stationary phase). After all (or most anyway) of the cells appear to be
quiescent I would like to reinduce growth again and monitor the production of
certain proteins via Western blotting etc.
What I want to ask is: Have any of you out there got any methods that you
like for doing just this sort of thing?
I dont think I need to make my cells arrest in any particular phase of the
cell cycle, I just want to be able to make them stop growing and then
re-initiate 'normal' growth.
Thanks for any info. in advance.
Dr. Simon Dawson TEL:+44 (0)115 9249924 ex. 44787
Dept. of Biochemistry FAX:+44 (0)115 9422225
Queens Medical Centre Email:Simon.Dawson at .nott.ac.uk
Clifton Boulevard http://www.ccc.nottingham.ac.uk/~mbzspd/Simon.html
U.K. "Back off man, I'm a scientist!" - Bill Murray.
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