Cell Culture Question
yacman at homer24
Sun Jan 28 23:31:06 EST 1996
Simon Dawson (Simon.Dawson at nott.ac.uk) wrote:
: A question about cell cuture techniques. Well, one technique in particular
: I have some Swiss 3T3 cells and I would like to make them go quiescent (i.e.
: stationary phase). After all (or most anyway) of the cells appear to be
: quiescent I would like to reinduce growth again and monitor the production of
: certain proteins via Western blotting etc.
: What I want to ask is: Have any of you out there got any methods that you
: like for doing just this sort of thing?
: I dont think I need to make my cells arrest in any particular phase of the
: cell cycle, I just want to be able to make them stop growing and then
: re-initiate 'normal' growth.
: Thanks for any info. in advance.
: Dr. Simon Dawson TEL:+44 (0)115 9249924 ex. 44787
: Dept. of Biochemistry FAX:+44 (0)115 9422225
: Queens Medical Centre Email:Simon.Dawson at .nott.ac.uk
: Clifton Boulevard http://www.ccc.nottingham.ac.uk/~mbzspd/Simon.html
: U.K. "Back off man, I'm a scientist!" - Bill Murray.
Try serum deprivation, either no serum or low serum.
L. Scot Bastian Ph.D. 206-762-1010 ext. 1918 (voice)
University of Washington at the 206-764-2689 (FAX)
Veterans' Affairs Medical Center yacman at u.washington.edu (Email)
1660 S Columbian Way South
Seattle WA 98108
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