Ethanol precipitations: disposable 30 ml tubes?
Harry J. Witchel
Harry.Witchel at bristol.ac.uk
Sun Jan 28 11:36:08 EST 1996
Greetings Ferocious Mages of Nucleic Acids--
I fear I have a contamination problem, so I would like to find a brand
of disposable tube which can be spun quickly. I fear that plasmids from
my maxipreps of probe plasmids may get into my RNA preps. I do all my larger
ethanol precipitations (> 15 mls) in a small set of 30 ml corex tubes I have.
I would like to use a disposable tube for these ethanol precipitations, but I
think that falcon blue tubes would fall to pieces at the speeds (12,000 rpm in
an SS-34 rotor) which I find useful for precipitating small quantities of DNA
(100 micrograms) out of 25 mls of solution. Is there an appropriate tube/spin
system for ethanol precipitations of larger volumes using disposable tubes.
I don't think that washing up is a solution to the problem, because
currently after using the corex tubes, I rinse them with water, soak them
overnight with 10% (v/v) HCl to the brim, then I wash the tubes normally in
soapy water etc. using a reserved set (ie post acid) of bottle brushes.
Finally the tubes are baked at 180C for 7 hours.
Harry.Witchel at Bristol.AC.UK
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