Need a rapid method to check transgenicity in mice? in mice

Gary K. gtk10583 at glaxo.com
Mon Jan 29 08:45:09 EST 1996


In article <1996Jan29.124016.3925 at news.huji.ac.il>, Mirta Grifman
<mirtag at leonardo.ls.huji.ac.il> wrote:

> In our lab the standard method is to tail the mice and extract DNA from 
> the tail and then PCR with primers for the transgene. The protocol seems 
> quite old-fashioned and takes too much time. Wouldn't it be possible to 
> cut some hairs and PCR from them? I would very much appreciate any help 
> on this.
> Mirta Grifman



Mirta,

There is a reference in Biotechniques (1991 Vol.10, No.4, pp.506-513) for
doing PCR on hair samples as well as other unusual samples.

Hope this helps,
Gary



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