Removing DNA from Slot Blotter

martin LEACH leach at bu.edu
Mon Jan 29 15:59:06 EST 1996


To be 100% sure...make up a DNAseI digestion mix and incubate it in the
wells...incubate...then rinse with a protein denaturing agent...or pace the
plexiglass slot blotter in an 80C oven for an hour or so...

Martin


Brian Margolin (margolin at darkwing.uoregon.edu) wrote:
: I need to use a slot blotter that has previously been used with large 
: amounts of plasmid DNA.  The plasmid DNA has unfortunately been 
: contaminating subsequent blottings.  What would be the best way to remove 
: the DNA from the plexiglass slot blotter?  Thanks for any info.  

: Brian

: --
: ------------------------------------------------------------------------------
: Brian Margolin					margolin at darkwing.uoregon.edu
: Institute of Molecular Biology					(541)346-5197
: University of Oregon			http://darkwing.uoregon.edu/~margolin
: Eugene, OR 97405

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