Removing DNA from Slot Blotter

[martin LEACH]

To be 100% sure...make up a DNAseI digestion mix and incubate it in the
wells...incubate...then rinse with a protein denaturing agent...or pace the
plexiglass slot blotter in an 80C oven for an hour or so...

Martin


Brian Margolin (margolin at darkwing.uoregon.edu) wrote:
: I need to use a slot blotter that has previously been used with large 
: amounts of plasmid DNA.  The plasmid DNA has unfortunately been 
: contaminating subsequent blottings.  What would be the best way to remove 
: the DNA from the plexiglass slot blotter?  Thanks for any info.  

: Brian

: --
: ------------------------------------------------------------------------------
: Brian Margolin					margolin at darkwing.uoregon.edu
: Institute of Molecular Biology					(541)346-5197
: University of Oregon			http://darkwing.uoregon.edu/~margolin
: Eugene, OR 97405

--
.....          Martin Leach                Email:leach at bu.edu
   _|____      Dept. of Pharmacology       Phone: (617) 638-5323
   / o  /      Boston Univ. School of Med. Fax:   (617) 638-4329
 _/  |-/__==/  80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118            "Not the old underpants
               USA                                on your
                                             head.....WIBBLE"

My homepage: http://155.41.115.114/Leach.html
Biotech company WWW REgistry has moved to: http://www.data-transport.com
Check out the Biotech Rumor mill: http://155.41.115.114/jumper/rumor.html