Ethanol precipitations: disposable 30 ml tubes?
walberg at simmons.swmed.edu
Mon Jan 29 09:23:38 EST 1996
> Harry.Witchel at bristol.ac.uk (Harry J. Witchel) wrote:
> >Greetings Ferocious Mages of Nucleic Acids--
> > I fear I have a contamination problem, I fear that plasmids from
> >my maxipreps of probe plasmids may get into my RNA preps.
> I would like to use a disposable tube for these ethanol precipitations,
> > I don't think that washing up is a solution to the problem, because
> >currently after using the corex tubes, I rinse them with water, soak them
> >overnight with 10% (v/v) HCl to the brim, then I wash the tubes normally in
> >soapy water etc. using a reserved set (ie post acid) of bottle brushes.
> >Finally the tubes are baked at 180C for 7 hours.
> > Many thanks,
> > Harry
> >Harry.Witchel at Bristol.AC.UK
One word- BLEACH.
More information about the Methods