Ethanol precipitations: disposable 30 ml tubes?
cwen at aux.btny.purdue.edu
Mon Jan 29 14:16:11 EST 1996
Harry.Witchel at bristol.ac.uk (Harry J. Witchel) wrote:
>Greetings Ferocious Mages of Nucleic Acids--
> I fear I have a contamination problem, so I would like to find a brand
>of disposable tube which can be spun quickly. I fear that plasmids from
>my maxipreps of probe plasmids may get into my RNA preps. I do all my larger
>ethanol precipitations (> 15 mls) in a small set of 30 ml corex tubes I have.
> I would like to use a disposable tube for these ethanol precipitations, but I
>think that falcon blue tubes would fall to pieces at the speeds (12,000 rpm in
>an SS-34 rotor) which I find useful for precipitating small quantities of DNA
>(100 micrograms) out of 25 mls of solution. Is there an appropriate tube/spin
>system for ethanol precipitations of larger volumes using disposable tubes.
> I don't think that washing up is a solution to the problem, because
>currently after using the corex tubes, I rinse them with water, soak them
>overnight with 10% (v/v) HCl to the brim, then I wash the tubes normally in
>soapy water etc. using a reserved set (ie post acid) of bottle brushes.
>Finally the tubes are baked at 180C for 7 hours.
> Many thanks,
>Harry.Witchel at Bristol.AC.UK
I had this problem before. However, I figured out that if the solution was separated into two 2-ml tubes and spin for 5min, then I only need to
spin 6 time and spend only 30min to spin down the DNA into two tubes.
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