Ethanol precipitations: disposable 30 ml tubes?

CK Wen cwen at aux.btny.purdue.edu
Mon Jan 29 14:16:11 EST 1996


Harry.Witchel at bristol.ac.uk (Harry J. Witchel) wrote:
>Greetings Ferocious Mages of Nucleic Acids--
>	
>	I fear I have a contamination problem, so I would like to find a brand 
>of disposable tube which can be spun quickly.  I fear that plasmids from 
>my maxipreps of probe plasmids may get into my RNA preps.  I do all my larger 
>ethanol precipitations (> 15 mls) in a small set of 30 ml corex tubes I have. 
> I would like to use a disposable tube for these ethanol precipitations, but I 
>think that falcon blue tubes would fall to pieces at the speeds (12,000 rpm in 
>an SS-34 rotor) which I find useful for precipitating small quantities of DNA 
>(100 micrograms) out of 25 mls of solution.  Is there an appropriate tube/spin 
>system for ethanol precipitations of larger volumes using disposable tubes.
>
>	I don't think that washing up is a solution to the problem, because 
>currently after using the corex tubes, I rinse them with water, soak them 
>overnight with 10% (v/v) HCl to the brim, then I wash the tubes normally in 
>soapy water etc. using a reserved set (ie post acid) of bottle brushes.  
>Finally the tubes are baked at 180C for 7 hours.  
>
>	Many thanks,
>	Harry
>
>Harry.Witchel at Bristol.AC.UK

I had this problem before.  However, I figured out that if the solution was separated into two 2-ml tubes and spin for 5min, then I only need to
spin 6 time and spend only 30min  to spin down the DNA into two tubes.




Chi-Kuang



















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