probs digesting plant genomic
GRANT,Patricia
BOTPG at LUFF.LATROBE.EDU.AU
Mon Jan 29 20:34:56 EST 1996
I'm trying to digest genomic DNA from mature leaf tissue for eventual ligation
and use in inverse-PCR. I have been having problems trying to get the
stuff to digest with the restriction enzymes of choice (PvuII or XhoI). I've
tried several methods of genomic isolation including a quick and dirty
arabidopsis method (thought its not arab I'm working on), then CsCl gradient
then CTAB. None of these produced DNA which would cut with either of the
enzymes. Finally I have included Qiagen columns (anion exchange) as the final
purification step and it now cuts when I use BamHI but won't with either of
the others. Does anyone have any suggestions....?
Thanks
Trish
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