RNAse without DNAse (low salt)?
lruble at bronze.ucs.indiana.edu
Tue Jan 30 10:27:59 EST 1996
After never seeing the problem for years, suddenly my new RNAse stock
(Sigma 95% grade) prepared by Sambrooks 10 minute "boil" apears
to have a DNAse activity which only manifests itself in low
salt restriction digests (NEB buffer #1, 10 mM Bis-tris propane).
Anyone else seen it?
Where else might be a good source of RNAse?
Washington University of St. Louis
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