Subcloning large PCR products

rosasg01 at rosasg01 at
Mon Jan 29 18:46:14 EST 1996


For the last 3 months I have tryed to subclone a 8kb PCR product. So
far, nothing has worked. Does somebody has done something like this???
I'm sure somebody must have done it. If you have successfully
subcloned large PCR products, please let me know how big it was and
the protocol that you used for both PCR and subcloning. I'll really
appreciate any suggestions.
German Rosas-Acosta, graduate student
NYU Med. Center

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