(fwd) Fluorescence-labelled biotin

[Mara Michelle Casar]

Lou Hom wrote... 
> Does anyone know of any fluorescent molecules that tolerate conjugation to
> biotin?  I'm wondering if there are any biotin-fluorophore-protein
> conjugates on record anywhere.  

I'm about to start feeding a protein to cells to see how it is 
internalized.  I've biotinylated the protein and am planning to track it 
using a fluorescently-labeled avidin derivative.  I haven't come across 
anything talking about fluorescently-labeled biotin, though.  You could 
check the Molecular Probes catalog - it has lots of info on this subject.

-Mara Casar
Cell and Molecular Biology Program
Duke University