------pure plasmid c LiCl---????
pkursula at cc.oulu.fi
Tue Jan 30 06:36:16 EST 1996
On 29 Jan 1996 gedmunds at kean.ucs.mun.ca wrote:
> I been given a plasmid purifying method using LiCl.
> Its looks easy and is quick that makes me wonder how effective
> it is of making pure plasmid.
> Anyone with any experience with this method?
I've used a method like that... it gives about 1 mg of PURE plasmid from
200 ml of bacterial culture, good enough for any experiments you would
want to make. I've done sequencing, subcloning, electroporation of
mammalian cells etc. with no problems whatsoever.
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