Removing DNA from Slot Blotter

Tom Thatcher ttha at uhura.cc.rochester.edu
Tue Jan 30 22:13:35 EST 1996


In <lruble.823014941 at bronze.ucs.indiana.edu> lruble at bronze.ucs.indiana.edu (the End) writes:


>>I need to use a slot blotter that has previously been used with large 
>>amounts of plasmid DNA.  The plasmid DNA has unfortunately been 
>>contaminating subsequent blottings.  What would be the best way to remove 
>>the DNA from the plexiglass slot blotter?  Thanks for any info.  

>>Brian

Soak for 1 hour in 0.1 N HCl, then rinse with ddH2O.
-- 
Tom Thatcher                          | You can give a PC to a Homo habilis,
University of Rochester Cancer Center | and he'll use it, but he'll use it
ttha at uhura.cc.rochester.edu           | to crack nuts.



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