fluorescence PCR

suihuang at usa1.com suihuang at usa1.com
Tue Jan 30 23:18:46 EST 1996


Does anybody has experience with visualizing PCR products 
(in agarose or PA gel) using 5' fluorescence labelled primers 
(Amersham) and the FluorImager (Molecular dynamics) ?
Especially, how sensitive is this method compared to 
32P-nucleotide incorporation ?

Thank you for any helpful comments.

Sui Huang
Children's Hospital, Boston




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