western blot stripping protocol
szsnoopy at chip.ucdavis.edu
Wed Jan 31 17:55:52 EST 1996
I am having problems stripping off my primary antibody off of my
western blots. I am using nitrocellulose and following NEN's stripping
protocol that calls for a 30 minute incubation in 62.5 mM Tris-HCL pH
6.8, 2% SDS, and 100 mM 2-mercaptoethanol at 50 degrees centigrade. My
results show that only the secondary antibody is being washed off and my
primary is still there. Any suggestions, comments or is there a better
protocol to remove the primary antibody off nitrocellulose?
Thanks in advance.
More information about the Methods