Methods January 1996 Archives by author

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Starting: Mon Jan 1 11:37:41 EST 1996
Ending: Wed Jan 31 23:29:05 EST 1996
Messages: 1505

PCR library using M13 and specific internal primer "Marianne Leverone ", BIO
Non-fat dry milk...really non-fat? "Marianne Leverone ", BIO
Does Ultrasound destroy dsDNA? "Marianne Leverone ", BIO
Electroporation nutrient requirements of E. coli "Marianne Leverone ", BIO
How many restriction sites fragments per genome?
syber green
troubles with PCR
Quantitating Northerns on MD PhosphorImager -
(none) user 3046
Help about FIAU Patricia Gleydes Morgante ib - bio 7548
Help with FIAU again... Patricia Gleydes Morgante ib - bio 7548
DNA dried to filter paper Francisco G da Nobrega ib - bio 7588
THOMAS Scientific Francisco G da Nobrega ib - bio 7588
northerns - sharp bands - SOLUTION, THANKS!! Francisco G da Nobrega ib - bio 7588
How to transfer plasmids???? Francisco G da Nobrega ib - bio 7588
northerns - sharp bands - SOLUTION, THANKS!! Francisco G da Nobrega ib - bio 7588
mRNA isolation problem K J C 944
directional cDNA cloning K J C 944
HELP. Narcissus tissue/cell culture media? at BBSRC.AC.UK
HELP!Tissue/cell culture media for Narcissus? at BBSRC.AC.UK
Narcissus cell/tissue culture media? at BBSRC.AC.UK
Narcissus tissue culture medium? at BBSRC.AC.UK
BrdU in immunoflourescence A.S.
Sequencing an oligo? ANTERO AIRAKSINEN
Quantification of Westerns AShaw5
Protein dimerisation in SDS-gel AShaw5
RACE Yasuhito Abe
In situ/ tubes Thermal Cycler? BIOSCI Administrator
BIOSCI miniFAQ, ver. 14-DEC-95 BIOSCI Administrator
(no subject) Adriana
RNA sequencing by RT Adriana
PCR from single colonies? Adriana
PCR from single colonies? Adriana
DGGE - help please Jason Affourtit
looking for CYTOKINE SCIENCES Keith Akama
need info on hygromycin S. Ali Alavizadeh
BsaBI digests M. Aleexeyeev
Antisense and sensibilities Bill Alexander
Static electricity and pipettes Bill Alexander
sequencing gels won't stick!! Bill Alexander
housekeeping gene rtpcr Bill Alexander
fresh transformations for protein expression?? M. Alexeyev
Looking 4 lon- E. Coli Mikhail Alexeyev
Protein Expression in E. coli Mikhail Alexeyev
Antisense and sensibilities Mikhail Alexeyev
restiction enzyme Mikhail Alexeyev
Antisense and sensibilities Mikhail Alexeyev
[HELP]Cloning double stranded oligo DNA fragment into vector. Mikhail Alexeyev
Antisense and sensibilities Mikhail Alexeyev
Antisense and sensibilities Mikhail Alexeyev
Antisense and sensibilities Mikhail Alexeyev
ligation of blunt PCR products Mikhail Alexeyev
Super broth protocol? Chen Ho An
DNA dried to filter paper Nicole Anderson
where to get antibody 9E10? Tom Anderson
Baculo equipment in Europe Tim Angelotti
Protein Crystallization Supplies Tim Angelotti
Protein Crystallization Supplier Tim Angelotti
PCR on whole blood Lo Wing Ip Anthony
Antisense and sensibilities Tracy Aquilla
Antisense and sensibilities Tracy Aquilla
Antisense and sensibilities Tracy Aquilla
Antisense and sensibilities Tracy Aquilla
Makes SENSE to me Tracy Aquilla
Antisense and sensibilities Tracy Aquilla
Antisense and sensibilities Tracy Aquilla
Quantitating Northerns on MD PhosphorImager Tracy Aquilla
ECL Northerns Tracy Aquilla
mRNA isolation problem Archambault, Christian
mRNA isolation problem Archambault, Christian
Non-fat dry milk...really non-fat? Curt Ashendel
CMV-selectable marker Graham Atherton
"Anchored" PCR problem Cathy Aversa
half-life of phosphorimager screen Juan A. Ayala
looking for anti-SulA antibodies Juan A. Ayala
Sequencing Kit required RAJESH BHOOLA
looking for carbinoxamin BIOSCIPA%IPV85.UNIPV.IT at earn-relay.ac.uk
Protein dimerisation in SDS-gel BOMINAAR at chem.rug.nl
In search of yeast plasmid Dan Baggott
resuspending intractable DNA Tim Barnett
Please help me to reamlify RAPD band Tim Barnett
DNA in agarose gels Tim Barnett
UNSUCCESFUL DIGESTION USING TO ENZYMES Tim Barnett
Cacodylate & CoCl2 Chris Barry
Cacodylate & CoCl2 Chris Barry
(test) Chris Barry
Looking for Avidin Beads Chris Barry
differential display Kirk A. Bartholomew
Help: potato DNA isolation Stefan Bartling
Concealed (Genequant II) L. Bastian
DNAse treatment of isolated total RNA ? L. Bastian
transfection HL-60 L. Bastian
Cell Culture Question L. Bastian
DNA precipitation Joe Bateman
superbroth Jose M. Bautista
pH of TE Jose M. Bautista
rt-pcr problems Jose M. Bautista
Protein Expression in E. coli Jose M. Bautista
HPLC systems Jose M. Bautista
Need Rabbit iNOS Sequence Evelyn Bayna
Need Rabbit iNOS Sequence Evelyn Bayna
JRH Bioscience in Europe ???? Cliff Beall
Lambda Exonuclease and Sequencing PCR Products Robin Beech
Agar replacement Brett Beitzel
BrdUTP in FISH Brett Beitzel
Glass wells needed Brett Beitzel
(no subject) Stephen Bell
Morph Site Directed Mutag. Protocol Stephen Bell
Promega Magic Michael Benedik
software needed Paul Benke
Western problems Per Bergman
Stubborn DNA during resuspension Per Bergman
ABI Sequencer problem Per Bergman
Cheap glass plates for ABI automated sequencer Per Bergman
Transgenic Handbook Michele Bernasconi
Transgenic Handbook Michele Bernasconi
Transgenic Handbook Michele Bernasconi
Transgenic Handbook Michele Bernasconi
Transgenic Handbook Michele Bernasconi
Transgenic Handbook Michele Bernasconi
gene targeting Bernhard.Redl at uibk.ac.at
gene targeting Bernhard.Redl at uibk.ac.at
DNA extraction from Algae??? Deborah Bernsen
Protein dimerisation in SDS-gel Tom Bickle
Double-stranded DNA Bernd R. Binder
differential display Display Systems Biotech
Tetracycline-free bovine serum Display Systems Biotech
Differential Display Kit Display Systems Biotech
Sequencing mRNA from variable regions Peggy L. Bishop
growth of plasmids in the Stable 2 strain of E. coli Ron Bishop
Help I need a little bit of cytokine Allen Black
grant & funding newsgroups/websites? Shaun D. Black
Nice collection of Biochem/Mol Biol Web sites Shaun D. Black
RNAse for phage preps Rainer Blaesius
Is linear DNA mandatory for PCR amplification ?? Volker Blaschke
WWW Medline Search? Volker Blaschke
C-DNA Vector recomendations? Dr. Leonard N. Bloksberg
ECL Northerns Darren Boehning
DNA stuck in agarose gel wells Darren Boehning
fresh transformations for protein expression?? Torsten Boerchers
Plasmid drawing software Torsten Boerchers
T7 transcribed RNA probes Torsten Boerchers
T7 transcribed RNA probes Torsten Boerchers
T7 transcribed RNA probes Torsten Boerchers
Does His-tag have to be terminal? Torsten Boerchers
request for pBS vector DNA sequence Torsten Boerchers
Quantitating Northerns on MD PhosphorImager Torsten Boerchers
T/A cloning Torsten Boerchers
differential display Russ Boggs
ligation of blunt PCR products Danuta Bois
The "megaprimer" method of PCR Eckhard Boles
Transferring High Percentage Sequencing Gels Reese Bolinger
E. coli strains Elizabeth M. Boris
THOMAS Scientific Joan Boyce
BsaBI digests Chris Boyd
EcoB supplier ? Chris Boyd
DEAE-transfection of EL-4 cells Paul Boyer
Where to Post Used Laboratory Equipment of Sale Scott K. Boyer
Non-fat dry milk...really non-fat? Alex Brands
need I-E k class II on single vector Melanie Brazil
Longe PCR problems. Rob Britton
upper limit to Inverse PCR products? Henry Broekhuyse
upper limit to Inverse PCR products? Henry Broekhuyse
eppendorf tube closures Tori Brophy
GFP Quantitation ??? Mr. A. Brown
HELP - best NOS assay Bruce C. Kone, M.D.
imaging gels Charles Brummer
DNA extraction from formaldehyde-preserved tissue Johan Burger
Nematode DNA extraction Johan Burger
Primer & probe design software? Bill Burnett
In situ oligo-probe design Bill Burnett
Mixed sequences Bill Burnett
DNA in agarose gels Bill Burnett
DNA isolation from crustaceans Bill Burnett
Inexpensive Balance Bill Burnett
Inexpensive Balance Dr. Bill Burnett
hns-E.coli Geraldine Butler
RNA preps. Stig Ove B¿e
gel mobilty shifts CDMENU at ccs1.cc.monash.edu.au
codon usage CDMENU at ccs1.cc.monash.edu.au
CV13 (NB) vector map Manuel G. CLAROS
Molecular Biology Software Manuel G. CLAROS
codon usage Manuel G. CLAROS
Recombinant DNA courses at Indiana University JANE CLAY
Fidelity DNA sequencing Mr. D.J. Cain
Is this newsgroup archived? Carolyn Calnan
Please help me to reamlify RAPD band Carolyn Calnan
T/A cloning Martin Cann
HEK 293 Library? Steven James Carlisle
Inexpensive Balance Charles Carter
Test questions for molec. biol? Charles Carter
(fwd) Fluorescence-labelled biotin Mara Michelle Casar
Looking for T7 gene6 exonuclease Graham Casey
DNA stuck in agarose gel wells Brandt Cassidy
RNA extraction from small samples Ed Castro
Methods of isolating RNA and DNA Ed Castro
what's an easy way to look for cell death? Ed Castro
Denaturation of RNA Ed Castro
Is linear DNA mandatory for PCR amplification ?? Ed Castro
Fluorometers Ed Castro
RNA extraction from < 50 mg Ed Castro
troubles with PCR Ed Castro
Homology Search Software? Ed Castro
Glutathione-HRP conjugate George W Chacko
FOR SALE:Wild inverted microscope and LKB ultramicrotome. Mingi Chang
Shortened products with pET vector Clinton Chapple
DNA extraction from Algae??? Charlotte
WWW Medline Search? Daniel S. Chen
NMDA in embryo Shao-Yu Chen
NMDA receptor knockout mouse needed Shao-Yu Chen
How much should I added? Robert Y. S. Cheng
DNAse treatment of isolated total RNA ? John Chesters
DNAse treatment of isolated total RNA ? John Chesters
Silver stain protocol John Chesters
(no subject) LAU Tze Chin
DNA purification from clinical samples LAU Tze Chin
Immunoprecipitation's theory Nikolai Chitaev
HIS-TAG Purification of Nuclear Proteins in Tissue Culture Nikolai Chitaev
Does His-tag have to be terminal? Nikolai Chitaev
differential display Lely Chow
T/A cloning Dr. Duncan Clark
Thermostable reverse transcriptase Dr. Duncan Clark
BsaBI digests Dr. Duncan Clark
T/A cloning again. To Kinase or Not Dr. Duncan Clark
M13 cloning problem Dr. Duncan Clark
T/A cloning again. To Kinase or Not Dr. Duncan Clark
BsaBI digests Dr. Duncan Clark
M13 cloning problem Dr. Duncan Clark
PCR using Us instead of Ts Dr. Duncan Clark
EcoB supplier ? Dr. Duncan Clark
codon usage Dr. Duncan Clark
Temperature sensitive chelator of Mg Dr. Duncan Clark
removing nucleic acids from coli extracts? Dr. Duncan Clark
Quickest method to visualize plasmid presence in bugs? Dr. Duncan Clark
In situ/ tubes Thermal Cycler? Atgie Claude
In situ/ tubes Thermal Cycler? Atgie Claude
Ethanol precipitations: disposable 30 ml tubes? Dr. J.P. Clewley
help with research project related to antibodies Pierce Chemical Co
M13RF DNA..gDNA contamination Duncan Cochran
Sense/Antisense nonsense Duncan Cochran
DNA stuck in agarose gel wells Wayne Coco
Please help!!! Roy Cohen
southern (discomfort) Shelley Cole
Has anyone used the Buffer Puffer? Shelley Cole
DNA to quantitate RNA Shelley Cole
Denaturation of RNA Debbie Colwell
DNA fixation on Agarose gel Pierre Commercon
Help about ETS 2 cDNA Pierre Commercon
syber green Computer
transgenic mouse cost!!!!! Richard Converse
grant & funding newsgroups/websites? Richard Converse
NeoR alternative vectors? CoreyKeith
anti-Bcl-2 antibody CoreyKeith
Carafate, Sucralfate CoreyKeith
More and more confused about making SENSE Joao Vasconcelos Costa
blocking in northern blots Doug Craig
Stratagene RAGE Controller Wanted George Cross
EGF ELISA Mickey Croyle
Tyrosine kinase detection. Fiona Culley
PCR on human tissues Fiona Culley
success with antisense oligos? Tim Cullingford
DNA extraction from sperm - method wanted Jim Cummins
ivf-dish Jim Cummins
Full-length PCR for mtDNA? Jim Cummins
Paraquat and sperm cells Jim Cummins
In vitro protein phosphorylation Mike Curtin
Isolating RNA for Diff. Display ?? Mike Curtin
Need reference for Diff. Display- from single cell Mike Curtin
phosphorylation state-specific antibodies Andrew J. Czernik
Is Ethanol ppt necessary following chloroform extraction MICHAEL DALRYMPLE
Subcloning large PCR products MICHAEL DALRYMPLE
ligation of blunt PCR products Eric DELABESSE
What is PRK7 vector? Eric DELABESSE
What is PRK7 vector? Eric DELABESSE
Lambda Screening Problems DOCON
UV Mutagenesis DSF
Anti-Mycoplasma agents DSF
Stubborn DNA during resuspension Richard Daines
Stubborn DNA during resuspension Richard Daines
DNA stuck in agarose gel wells Richard Daines
help for QIAGEN (QIAprep Spin) Richard Daines
T/A cloning again. To Kinase or Not Michael Dalrymple
southern (discomfort) David L. Haviland, Ph.D.
Peptide immobilisation - spacer length? Michael Daws
NeoR alternative vectors? Michael Daws
[HELP]Cloning double stranded oligo DNA fragment into vector. Michael Daws
[HELP]Cloning double stranded oligo DNA fragment into vector. Michael Daws
[HELP]Cloning double stranded oligo DNA fragment into vector. Michael Daws
Chemiluminescence Michael Daws
Chemiluminescence Michael Daws
College Experiment Elliott Dawson
BsaBI digests Elliott Dawson
Phage Stocks Elliott Dawson
BsaBI digests Elliott Dawson
Fluorescence-labelled biotin Elliott Dawson
Chromosomal location of muscarinic receptor genes Elliott Dawson
Desperately seeking Elliott Dawson
rt-pcr problems Simon Dawson
Sequencing problem Simon Dawson
pH of TE Simon Dawson
RT-PCR quantification controls Simon Dawson
T/A cloning Simon Dawson
Sequencing query Simon Dawson
T/A cloning Simon Dawson
Cell Culture Question Simon Dawson
lambda phage reprise Oliver S. DePeyer
Protein pI and migration on an SDS-PAGE gel Todd DeZwaan
Yeast Colony PCR Dan Deaguiar
Stubborn DNA during resuspension Graham Dellaire
New URL and Third Announcement: Conference on Eukaryotic DNA replication Graham Dellaire
Membranes, swapping, characteristics R. Rex Denton
cap independent expresion Bart Depuydt
Fidelity of LA-PCR Brian Desany
Troubles with Pfu polymerase Brian Desany
Stubborn DNA during resuspension M.T. Dettori
PCR from blood samples Linda DiPersio
In vitro protein phosphorylation Eddie Diehl
Quantification of Westerns Eddie Diehl
Phosphorescent Ink Eddie Diehl
Looking 4 lon- E. Coli Digs
monovalent streptavidin Digs
Hydroxylamine for cleaving proteins? Digs
genomics with SalI or AatII? John Dixon
Bgal epitope on paraffin embedded sections? John Dixon
Lipofecting mouse ES cells toxic? John Dixon
Alternatives to amber codon? John Dixon
CRE protein John Dixon
Quickest method to visualize plasmid presence in bugs? John Dixon
Synthesizing RNA Andrew Dizon
rat cDNA estrogen receptor Guivarc_h Dominic
pH of TE Dominique
Taq-Man anyone? Peter Doris
Looking 4 lon- E. Coli Tom Dougherty
Question: lambda plaques not transparent, bacterial lawn not opaque Tom Dougherty
Any CsCl pure method help Harold Drabkin
DNA fixation on Agarose gel Tomas Drgon
Plasmid drawing software Antonio Duarte
Retroviruses and viral vectors Matthew Dubeck
Idea for Potential HIV Cure Matthew Dubeck
SOE PCR woes Ido Dubrawsky
Colorimetric assay for alkaline phosphatase (SEAP) Yves Durocher
nitroso-guanidine (NG) protocol? Scott Earley
soluble protein expression in E. coli Mad Dan Eccles
Anti-GST antibodies source? Mad Dan Eccles
Carbon dioxide-independent medium George F Edick
UNSUCCESFUL DIGESTION USING TO ENZYMES Truls Eide
Removing DNA from Slot Blotter the End
The "megaprimer" method of PCR the End
RNAse without DNAse (low salt)? the End
RNAse without DNAse (low salt)? the End
Protein dimerisation in SDS-gel Lutz Essers
Protein dimerisation in SDS-gel Lutz Essers
PLASMID COPY NUMBER DETERMINATION??? FG413
plasmid copy number in E. coli FG413
50-80 bp size marker Glibert Fabrice
Stubborn DNA during resuspension Tom Fagan
RT-PCR PROBLEMS Patrick HJ Falckh
PCR on whole blood Patrick HJ Falckh
Problems with RT-PCR Patrick HJ Falckh
Problems with RT-PCR Patrick HJ Falckh
PCR and DMSO Patrick HJ Falckh
Sequencing mRNA from variable regions Patrick HJ Falckh
P32 or Chemiluminescence, Biotin Probes for library screening Patrick HJ Falckh
How to 32P label a PCR probe? Patrick HJ Falckh
ARGH! Northern Problems (again!) Patrick HJ Falckh
Quantitating Northerns on MD PhosphorImager Patrick HJ Falckh
oligonucleotide probe design Patrick HJ Falckh
Longe PCR problems. Patrick HJ Falckh
Indented Replies Patrick HJ Falckh
PCR from blood samples Patrick HJ Falckh
Is Insect 28 and 18S banding different ? Patrick HJ Falckh
RNA extraction from < 50 mg Patrick HJ Falckh
Need a rapid method to check transgenicity in mice? in mice Patrick HJ Falckh
(no subject) Patrick HJ Falckh
TaqStart -hot start reagent Patrick HJ Falckh
TaqStart hot-start reagent Patrick HJ Falckh
TaqStart hot-start reagent Patrick HJ Falckh
Lysing Yeast Mark Farinha
SSCP Scot Federman
Subcloning large PCR products Luc Ferrari
HIS-TAG Purification of Nuclear Proteins in Tissue Culture S. Findley
T/A cloning Mike Finney
Expression of fusion proteins w/ GIII MD. Finucane
[HELP]Cloning double stranded oligo DNA fragment into vector. Karl Fischer
ARGH! Northern Problems (again!) Robert Fisher
PCR fidelity Micheal FitzGerald
DNA stuck in agarose gel wells Tim Flanigan
Chromosomal location of muscarinic receptor genes David Fogarty
HELP!!! Getting nothing but smears on sequencing gels. Marc Forestier
(none) Francis, Linda
Li-Cor vs ABI Sequencers Greg Fransen
test Ahnders Franzen
Restriction enzymes Rita Frassanito
restriction enzymes Rita Frassanito
Need reference for Diff. Display- from single cell Freddy
DD primers Freddy
RNA extraction from < 50 mg Freddy
POSTDOCTORAL POSITION AVAILABLE Freistadt, Marion
POSTDOCTORAL POSITION AVAILABLE Freistadt, Marion
PCR from single colonies? Christian Fritze
PCR from PAA gels? G
Protein interaction studies - co-expression in bacteria? G.P.
Prolonged radio-labeling with 35S-Methionin - any effect? G.P.
tris barbatol STEVEN GERHARDT
probs digesting plant genomic GRANT,Patricia
NAD Pyrophosphorylase GREENL1
Still problems detecting the Oct-1 Protein .... GST
PCR fidelity Warren Gallin
fixation-sensitive antibody Warren Gallin
Electroporation nutrient requirements of E. coli Greg Galloway
(no subject) Inmaculada Garcia
extractin mtDNA from fish scales Michael Gatt
Stripping Northerns? Dan Gebow
Homology Search Software? Dan Gebow
plasmid miniprep kit eval R. Dana - Green Gene
Plasmid Miniprep Web Site R. Dana - Green Gene
PCR on whole blood R. Dana - Green Gene
Reagents R. Dana - Green Gene
soluble protein expression in E. coli Saverio Brogna Genetics
BsaBI digests Szatmari George
Help in detecting oct-1 Gerhard
Sources Western Blot/ELISA Kits David J. Gerrick
What do *YOU* call background? Gil
Protein half - life determination? Dr R.V. Giles
Quantitating Northerns on MD PhosphorImager David Glover
Help - FESMID. Edmond Godfroid
Yeast Colony PCR Steven Goldberg
Photographic Paper Replacement for X-ray Film? Hugh Goldie
Transferring High Percentage Sequencing Gels Marc Goldstein
fresh transformations for protein expression?? David Goodin
E. coli nitroreductase S.D. Gorman
Question on how to radio-label a protein. Mark Gosink
HELP: purify protein from PAGE gel Gourley, Tania Suzanne
(no subject) Anton Scott Goustin
GFP mutant Anton Scott Goustin
Cell Culture Question Anton Scott Goustin
Pharmacia e-mail address Anton Scott Goustin
Best nylon membrane? James Graham
Membrane choice & spurious hybridization? James Graham
pH of TE Richard P. Grant
PCR fidelity Chris Greene
UK Supplier of BIO 101 products T. S. Grewal
SSCP Andy Grierson
The "megaprimer" method of PCR Andy Grierson
Antibody Crossreactivity???? Todd Griffith
Need a rapid method to check transgenicity in mice? in mice Mirta Grifman
pH of TE Duke Groebe
Non-fat dry milk...really non-fat? Duke Groebe
T7 transcribed RNA probes Duke Groebe
the most outrageous ripoff Duke Groebe
Sense-Antisense, genuine confusion Duke Groebe
lambda plaques RPA Groot
Question: lambda plaques not transparent, bacterial lawn not opaque RPA Groot
DNA fixation on Agarose gel Mike Gruidl
What to tag 5' ends for cytometry??? Todd A. Guillory
where to get antibody 9E10? Xin Guo
Help with nuclear runoff assay Swati Gupta
Lactose analogs Reinfried Götz
Sense/Antisense debate Ferland Louis H.
Antisense and sensibilities Ferland Louis H.
Antisense and sensibilities Ferland Louis H.
Makes SENSE to me Ferland Louis H.
Losing our SENSEs Ferland Louis H.
Antisense and sensibilities Ferland Louis H.
Antisense and sensibilities Ferland Louis H.
Sense/Antisense debate Ferland Louis H.
Sense/Antisense nonsense Ferland Louis H.
restiction enzyme Ferland Louis H.
Makes SENSE to me Ferland Louis H.
Losing our SENSEs Ferland Louis H.
Carrier DNA inhibits transfection Ferland Louis H.
Quantitating Northerns on MD PhosphorImager Ferland Louis H.
Antisense and sensibilities Ferland Louis H.
Antisense and sensibilities Ferland Louis H.
More and more confused about making SENSE Ferland Louis H.
Antisense and sensibilities Ferland Louis H.
Antisense and sensibilities Ferland Louis H.
Antisense and sensibilities Ferland Louis H.
Antisense and sensibilities Ferland Louis H.
Sense/Antisense nonsense Ferland Louis H.
SSCP Ferland Louis H.
Antisense and sensibilities Ferland Louis H.
Antisense and sensibilities Ferland Louis H.
request for pBS vector DNA sequence Ferland Louis H.
Ethanol precipitations: disposable 30 ml tubes? Ferland Louis H.
PCR polymerases N. van Belzen at HEMA
Anyone doing Maxam-Gilbert chemistry?-help! HPOLI
Concealed John Hachey
flourescent marker pen source? John Hachey
GeneQuant-Summary of responses John Hachey
TF families Allison Haggarty
Shortened products with pET vector Haitao
DNA purification from yeast Michael Hamilton
Li-Cor vs ABI Sequencers Robert G. Hamilton
Non-radioactive sequencing methods Robert G. Hamilton
DNA Isolation from whole blood Sabine Hanelt
monovalent streptavidin R Hanemaaijer
GFP mutant Richard R. Hardy
half-life of phosphorimager screen Kurt C. Harris
Locate Kikuya Kato? Bob Harrison
Any CsCl pure method helpful hints? Harry
RACE Harry
Savant 'fuge safety Steve Hartson
Shortened products with pET vector MV. Hayes
help: c-fos probe Lihua He
fresh transformations for protein expression?? JG. Head
NeoR alternative vectors? Jonathan Hecht
T/A cloning again. To Kinase or Not Jonathan Hecht
Know company? Amos Heckendorf
PCR and polymerase. Paul N Hengen
superbroth Paul N Hengen
Antisense and sensibilities Paul N Hengen
DNA from agarose - home made spin columns? Paul N Hengen
Tropilon Plus Membrane SWAP Update Paul N Hengen
Sense/Antisense debate Paul N Hengen
Need info re DNA-spec. dyes Paul N Hengen
M13 cloning problem Paul N Hengen
Plasmid Miniprep Web Site Paul N Hengen
magnetic bead losses during manipulation Paul N Hengen
Sequence of pAS1 is available Paul N Hengen
syber green Paul N Hengen
M13 cloning problem Paul N Hengen
Is this newsgroup archived? Paul N Hengen
Temperatures for precipitation of DNA Paul N Hengen
magnetic bead losses during manipulation Paul N Hengen
Looking for Jo\l Baguet Paul N Hengen
request for pBS vector DNA sequence Paul N Hengen
Promega Magic Paul N Hengen
request for pBS vector DNA sequence Paul N Hengen
Antisense and sensibilities Paul N Hengen
magnetic bead losses during manipulation Paul N Hengen
Promega Magic Paul N Hengen
sequencing gels won't stick!! Paul N Hengen
Warning! More Membrane SWAPs Paul N Hengen
DNA purification from clinical samples Paul N Hengen
CG-Repeats in Cycle Sequencing Lars Hesse
Pichia Expression Meeting David R. Higgins
rt-pcr problems Jody K. Hirsh
Promega minipreps crapping out Jody K. Hirsh
Suggestion (Re: fresh transformations for protein expression??) Jody K. Hirsh
DEAE-transfection of EL-4 cells Hung-yiu Ho
BrdU in immunoflourescence Hung-yiu Ho
fresh transformations for protein expression?? Flip Hoedemaeker
PCR using Us instead of Ts Les Hoffman
X-ray film developers Marcel Hoffmann
Sequencing an oligo? Marcel Hoffmann
Oligotex from Qiagen vs column Angela Hofstra
cell Death ELISA Angela Hofstra
Why is it _glacial_ acetic acid??? and NaAc question Susan Jane Hogarth
Why is it _glacial_ acetic acid??? and NaAc question Susan Jane Hogarth
Inexpensive Balance Susan Jane Hogarth
antibody against human ribosomal proteins Andreas Hohlbaum
Fluorescence-labelled biotin Louis Hom
CapFinder PCR cDNA Library Construction Kit Tatja Hopman
Looking 4 lon- E. Coli Mark Horn
hot start beads Robert Horton
long pcr Robert Horton
Please help!!! Robert Horton
Antisense and sensibilities Robert Horton
transgenic mouse cost!!!!! Robert Horton
Antisense and sensibilities Robert Horton
DNA from agarose - home made spin columns? Robert Horton
Sense/Antisense debate Robert Horton
Losing our SENSEs Robert Horton
Sense/Antisense debate Robert Horton
Molecular Biology Software Robert Horton
PCR on whole blood Robert Horton
T/A cloning Robert Horton
Antisense and sensibilities Robert Horton
Antisense and sensibilities Robert Horton
Sequencing mRNA from variable regions Robert Horton
PCR from single colonies? Robert Horton
PCR-Ligation-PCR gene fusion protocol Robert Horton
Inexpensive Balance Robert Horton
SOE-PCR woes! Robert Horton
Anyone doing Maxam-Gilbert chemistry?-help! Robert Horton
Makes SENSE to me Ken Howe
Losing our SENSEs Ken Howe
Quick RNA prep methods needed Ken Howe
Sense-Antisense, genuine confusion Ken Howe
Please help me to reamlify RAPD band Bixing Huang
capped or not capped RNA transcript? IJ Huang
DNA fixation on Agarose gel IJ Huang
help: Northwestern blot assay IJ Huang
q: stability of 32p-RNA IJ Huang
HEK 293 cells Trish Hurley
Neuronal cell culture Jim Hutchins
Blocking Phosphotyrosine Blots Jim Hutchins
regions downstream from p53 gene anyone??? The doctor is IN.
Looking for topoII inhibitor The doctor is IN.
REPOST: Somatic cell hybrids Kanematsu Institute
Tiny amount mRNA Yutaka Ishiwatari
Pst I clone source? J.Carnwath
Non-radioactive sequencing methods JLCruise
DNA fixation on Agarose gel Daniel Jacobs
genomic library for seq DNA with high A+T contents Asfar Jamel
T/A cloning Alex C. Jeffries
Non-fat dry milk...really non-fat? Csaba Jeney
Recent mutator E. coli strains related to MutD phenotype? Csaba Jeney
fresh transformations for protein expression?? Csaba Jeney
DNAse treatment of isolated total RNA ? Teis Jensen
Antisense and sensibilities Jfloring at aol.com
Genequant Jfloring at aol.com
Phosphoimager Changcun Jia
CRE protein William Jia
How to 32P label a PCR probe? Jim
syber green Jim
one day symposium on gene expression and mutation analysis Li Jin
freezing mixtures Federal Job
College Experiment JohnT6020
Where to Post Used Laboratory Equipment of Sale Mark Johnson
Ethanol precipitations: disposable 30 ml tubes? (Dave Johnston)
sequencing gels won't stick!! (Dave Johnston)
sequencing gels won't stick!! (Dave Johnston)
Antisense and sensibilities Mick Jones
More and more confused about making SENSE Mick Jones
Would you use high thruput sequencer? Mick Jones
Antisense and sensibilities Zophonias O. Jonsson
sequencing gels won't stick!! Zophonias O. Jonsson
removing nucleic acids from coli extracts? Zophonias O. Jonsson
Hydroxyapatite, how does it work? Zophonias O. Jonsson
DNA in agarose gels Rob Jordan
DNA prep from Rubus for PCR? Juan Jordano
Stripping Northerns? Joris.Winderickx at bio.kuleuven.ac.be
Stripping Northerns? Joris.Winderickx at bio.kuleuven.ac.be
Dihydrofolate synthesis? Fourie Joubert
DGGE Primers and GC clamp Juck, David
test Gary K.
test Gary K.
Need a rapid method to check transgenicity in mice? in mice Gary K.
Cosmid vectors question KONSTANTIN.KANYUKA at bbsrc.ac.uk
Plant genomic DNA extraction protocol? Chris Kafer
DNA isolation from crustaceans Ari Kahn
troubles with PCR Ari Kahn
Need a rapid method to check transgenicity in mice? in mice Steve Kain
any ftp sie with protein gif files Ali Karami
PCR from single colonies? David Karaolis
Rediprime labelling system David Karaolis
Meningococcus David Karaolis
Problems with RT-PCR Doris Karbach
How to transfer plasmids???? Peter Kaub
NeoR alternative vectors? Alan Kay
Zeomycin=neomycin? Alan Kay
Hydroxylamine for cleaving proteins? Alan Kay
DNA extraction from sperm - method wanted Kay, Graham William
Is Insect 28 and 18S banding different ? Nigel Kenward
syber green Rich Kernan
upper limit to Inverse PCR products? Michael Kertesz
How to transfer plasmids???? Junhyong Kim
P32 or Chemiluminescence, Biotin Probes for library screening Michael R. King
Has anyone used the Buffer Puffer? Kirsten
Coating-pH ? Svend Kjaer
linkers in bacterial expres? Svend Kjaer
Sense/Antisense nonsense Dima Klenchin
Sense/Antisense nonsense Dima Klenchin
Inexpensive Balance Dima Klenchin
Protein dimerisation in SDS-gel Dima Klenchin
Silver stain protocol Dima Klenchin
Anti-GST antibodies source? Dima Klenchin
Why is it _glacial_ acetic acid??? and NaAc question Dima Klenchin
Transforming ssDNA into JM 109 Dima Klenchin
Does Ultrasound destroy dsDNA? Stefan Kley
PCR of cDNA insert in pUC 18 Stefan Kley
request for pBS vector DNA sequence Stefan Kley
Looking for T7 gene6 exonuclease Donna C Knauber
Non-fat dry milk...really non-fat? Knepper, Marc A
DNA precipitations Knepper, Marc A
Gel analysis using the GelCompar program Roland Koelliker
What's an easy way to create free radicals in plant cells Sami Kohan
pg+host 5 shuttle vector Martin Kohlstock
T/A cloning Helen Korsmo
Quantitating Northerns on MD PhosphorImager Jarkko Kortesmaa
Where to Post Used Laboratory Equipment of Sale K.G. Korth
syber green K.G. Korth
Which kit for cleaning PCR products? Branislav Kovacech
PCR library using M13 and specific internal primer Alexander Kraev
genomics with SalI or AatII? Alexander Kraev
Anyone doing Maxam-Gilbert chemistry?-help! Alexander Kraev
Commercial pre-made Notherns Alexander Kraev
Maxam-Gilbert chemistry today ( a summary) Alexander Kraev
Which kit for cleaning PCR products? Alexander Kraev
fresh transformations for protein expression?? Cornelius Krasel
fresh transformations for protein expression?? Cornelius Krasel
fresh transformations for protein expression?? Cornelius Krasel
RNA isolation from blood Robin Kroone
His-Tag Ab??? Dr. Arthur L. Kruckeberg
fresh transformations for protein expression?? Dr. Arthur L. Kruckeberg
Antibodies to E2F-1 Stuart Kuhstoss
Pcr primers Els Nois del LAB
Biotech Rumour Mill Facelift martin LEACH
Any CsCl pure method helpful hints? martin LEACH
Pharmacia WWW site open! martin LEACH
Antisense and sensibilities martin LEACH
Pharmacia WWW site open! martin LEACH
Where to Post Used Laboratory Equipment of Sale martin LEACH
Ultra-low freezer: your choice martin LEACH
UK Supplier of BIO 101 products martin LEACH
ligation of blunt PCR products martin LEACH
How to 32P label a PCR probe? martin LEACH
THOMAS Scientific martin LEACH
T/A cloning martin LEACH
Boehringer WWW Site Open martin LEACH
looking for CYTOKINE SCIENCES martin LEACH
T7 transcribed RNA probes martin LEACH
RNA sequencing by RT martin LEACH
Email address of R & D of Amersham, UK martin LEACH
what's an easy way to look for cell death? martin LEACH
Sensitivity of Luciferase Assay martin LEACH
Pharmacia e-mail address martin LEACH
Removing DNA from Slot Blotter martin LEACH
Warning! More Membrane SWAPs martin LEACH
Use a genomic clone to find the cDNA martin LEACH
Use a genomic clone to find the cDNA martin LEACH
Colorimetric assay for alkaline phosphatase (SEAP) martin LEACH
DNA to quantitate RNA martin LEACH
Carbon-dioxide independent medium kLAUS LEHNERT
Shortened products with pET vector LSolo77049
Lysing Yeast LSolo77049
FIDELITY DNA Sequencing System Dean Danner Lab
Source for HeLa nuclei sought The Wagner Lab
TF families The Wagner Lab
DNA stuck in agarose gel wells John Ladasky
Lafmid BA vector Kevin J. Laddison
GST-fusion protein purification Mike Landon
PCR from single colonies? Nathan Lange
sequencing gels won't stick!! Tim Lansing
A thermostable DNA polymerase with a high processivity? Flemming Larsen
D1S80 Ladder-help? Garry P. Larson
Antisense and sensibilities Stephen R. Lasky
superbroth Stephen R. Lasky
Antisense and sensibilities Stephen R. Lasky
Makes SENSE to me Stephen R. Lasky
Losing our SENSEs Stephen R. Lasky
Antisense and sensibilities Stephen R. Lasky
Antisense and sensibilities Stephen R. Lasky
More and more confused about making SENSE Stephen R. Lasky
More and more confused about making SENSE Stephen R. Lasky
Antisense and sensibilities Stephen R. Lasky
Antisense and sensibilities Stephen R. Lasky
Antisense and sensibilities Stephen R. Lasky
Antisense and sensibilities Stephen R. Lasky
Phosphorescent Ink Stephen R. Lasky
Question: lambda plaques not transparent, bacterial lawn not opaque Stephen R. Lasky
Q: Separation of 12bp ds-oligo from ss Lcuster
sequencing gels won't stick!! Lcuster
the most outrageous ripoff Judy Leatherman
Press Release: A new Gel Imaging software package Blaine Leckett
subscribe Byeong-ha Lee
subscribe Byeong-ha Lee
Please, help me Jae-Seong Lee
Is linear DNA mandatory for PCR amplification ?? Jang Y Lee
[HELP]Cloning double stranded oligo DNA fragment into vector. Jimmy Lee
commerical suppliers DEVD-CMK or YVAD-CMK Kyung-Kwon Lee
supplier of DEVD-CMK or YVAD-CMK Kyung-Kwon Lee
supplier of DEVD-CMK or YVAD-CMK Kyung-Kwon Lee
supplier of DEVD-CMK or YVAD-CMK Kyung-Kwon Lee
Promega minipreps crapping out Philippe Lefebvre
DD primers Philippe Lefebvre
Ultra-low freezer: your choice Victor Levenson
Low temperature freezer Victor Levenson
Commercial pre-made Notherns Victor Levenson
what's an easy way to look for cell death? Michael Levin
Carrier DNA inhibits transfection David N. Levy
SOE-PCR woes! David N. Levy
(none) David N. Levy
SOE-PCR woes! David N. Levy
SOE PCR woes David N. Levy
WWW Medline Search? Eric W. Linton
Meicelase Zhan-Bin Liu
fluorescence PCR Deborah B. Loeb
96-channel pipette Mark Logan
HPRT Benke/Belmar Login
Positions available in palnt bioinformatics - advert3.dos [1/1] David Lonsdale
fresh transformations for protein expression?? Frank van de Loo
RNA isolation and cDNA synthesis Helena Lorenzo
lambda phage reprise LouPass
DNA in agarose gels LouPass
commercial suppliers of slides for in situ hybridization 2 Jukka Luoma
fresh transformations for protein expression?? Jennifer Lyon
PCR from single colonies? Jennifer Lyon
fresh transformations for protein expression?? Jennifer Lyon
Anti-sense oligonucleotides M.D.Barker
Cacodylate & CoCl2 Marie Helene DURRENS MPI
pBELO BAC11 vector prep Marie Helene DURRENS MPI
Plasmid drawing software Marie Helene DURRENS MPI
How many restriction sites fragments per genome? Marie Helene DURRENS MPI
5'oligolabelling kit ? Marie Helene DURRENS MPI
capfinder NEAL MUSTO
How do you... MacBoyee
Hydroxylamine for cleaving proteins? Marcia MacDonald
Biotinylating peptides Marcus Macht
In vitro protein phosphorylation Giovanni Maga
Balb/c genomic library Dixie Mager
PC12 cDNA library Pam Maher
Need polyamide tlc plates to sep dansyl AA - need vendor Malaleuca
More and more confused about making SENSE Rafael Maldonado
How many restriction sites fragments per genome? Rafael Maldonado
UV Mutagenesis Rafael Maldonado
mRNA stability in E. coli. Rafael Maldonado
T/A cloning again. To Kinase or Not Rafael Maldonado
T/A cloning again. To Kinase or Not Rafael Maldonado
southern (discomfort) Rafael Maldonado
Question: lambda plaques not transparent, bacterial lawn not , opaque Rafael Maldonado
How to transfer plasmids???? Rafael Maldonado
ivf-dish Titta Malinen
No subject Mangiafatsuma
Q: soluble marker enzymes Mangiafatsuma
directional cDNA cloning Ned Mantei
[HELP]Cloning double stranded oligo DNA fragment into vector. Ned Mantei
T/A cloning again. To Kinase or Not Francois-Xavier Maquart
Removing DNA from Slot Blotter Brian Margolin
Quickest method to visualize plasmid presence in bugs? Rolf Marteijn
In-Vitro Mutagenesis of BACs, PACs or Cosmids - any Suggestions ? Martin_Messerle
western blot stripping protocol Anthony Martinez
Phosphorescent Ink Matthew P. Frosch, M.D., Ph.D.
Help! total RNA from blood/leukocytes? Maureen_L_Ho
Clone Manager W.J. Mawby
Biotinylating peptides Brian D. McCabe
PCR from single colonies? Ian J. Mehr
Transfection of BHK and CHO cells Ralph Melcher
Hydroxylamine for cleaving proteins? Kojo Mensa-Wilmot
sorry - wrong henry Vivian Miao
upper limit to Inverse PCR products? Vivian Miao
southern (discomfort) Vivian Miao
PFGE trouble>Hi there, > My problem with my PFGE apparatus is I am getting streaks instead of bands. I believe that this is related to the fact that my buffer is giving slightly lower mA readings (18 instead of 21; 15 instead of 18) than usual. I have re-made the buffer exactly the same way that I always do when I get high quality results. I sent the power source in for repair, as I did 6 months ago when the same thing happened, and they found the the readings were slightly HIGH and re-calibrated the power supply. Since I got the power supply back, I have run a 24 hour gel with standards in 0.5 X TBE that came out OK. The normal gel that I am running, with 10 times less EDTA hence, 0.5 X TBe, has resulted in a continuous smear. >I also ran a PFGE gel on another machine, in another lab, with THE SAME buffer from the THE SAME bottle, and it came out well. Thank you, in advance, for your help. nkh@sage.purdue.edu Vivian Miao
magnetic bead losses during manipulation Mibi
upper limit to Inverse PCR products? Ted Michelini
the most outrageous ripoff Ted Michelini
DNA from agarose - home made spin columns? Michelle.Gleeson at SMTPGWY.AGRIC.NSW.GOV.AU
plant tissue grinding equipment Michelle.Gleeson at SMTPGWY.AGRIC.NSW.GOV.AU
T/A cloning Michelle.Gleeson at SMTPGWY.AGRIC.NSW.GOV.AU
Kleco pulveriser Michelle.Gleeson at SMTPGWY.AGRIC.NSW.GOV.AU
quickest method to visualize plasmid presence in bugs? Michelle.Gleeson at SMTPGWY.AGRIC.NSW.GOV.AU
eppendorf tube closures Michelle.Gleeson at SMTPGWY.AGRIC.NSW.GOV.AU
Phosphorescent Ink David Micklem
T/A cloning again. To Kinase or Not David Micklem
------pure plasmid c LiCl---???? David Micklem
Membranes, swapping, characteristics Microplate at eworld.com
Looking for Avidin Beads Microplate at eworld.com
Boehringer WWW Site Open Microplate at eworld.com
Warning! More Membrane SWAPs Microplate at eworld.com
Any experience with Thioredoxin Fusion? Charles A Miller
Purifying with Talon? Charles A Miller
Best ECL method? Charles A Miller
How do you develop X-ray film? Charles A Miller
Which kit for cleaning PCR products? Charles A Miller
Carafate, Sucralfate Ken Minneman
transfection HL-60 Ugo Moens
Sense/Antisense debate Richard Moldwin
Sense/Antisense nonsense Richard Moldwin
northerns - sharp bands Adrian Molenaar
Gel Reading Software? Barry Moore
SOE PCR woes Michael Morales
Plasmids from Bacillus thuringiensis Mireille Moulard
Fluorescent labeling of DNA Dr F Munkonge
Fluorescent labeling of DNA Dr F Munkonge
Commercial Western Blots Brian J Murphy
Pharmacia WWW site open! Bernard Murray
Antisense and sensibilities Bernard Murray
HELP - best NOS assay Bernard Murray
T/A cloning Bernard Murray
BsaBI digests Bernard Murray
BsaBI digests Bernard Murray
GFP mutant Bernard Murray
ras, raf-1, GAP clones wanted Bernard Murray
Automatic DNA sequencing: what's the best? N.K.Sankaran
Question on how to radio-label a protein. N.P.Dantuma at biol.ruu.nl
arabinose analog needed ELAINE B S NEWMAN
FRENCH DNA sequencing service Viguerie Nathalie
Plasmid drawing software Rick Neubig
GFP mutant Rick Neubig
Help: RNA preparation: very low yields with spinal cord; pH influence? Quoc Thang Nguyen
Omega-Cam Plasmid? Brad Nicholson
upper limit to Inverse PCR products? Brad Nicholson
upper limit to Inverse PCR products? Brad Nicholson
CAT Assay blues Soeren Jensby Nielsen
RT-PCR PROBLEMS Pamela Norton
sequencing long range PCR products Jamie Nourse
Help!! :) Primary Cultures of Mouse Hepatocytes Fatima Nunez
TriZol results? Lauryl Maiya J Nutter
Purifying with Talon? Yasushi OKADA
Does His-tag have to be terminal? Yasushi OKADA
Antibodies to His tag. Yasushi OKADA
Transferring High Percentage Sequencing Gels Litta Olsen
request for pBS vector DNA sequence Francis Ouellette
Source of ss eukaryotic DNA Jim Owens
adress of GENOME SYSTEMS Jim Owens
RACE PC16 at
restiction enzyme PC16 at
Melanin Extraction PRETORIUS
fixation-sensitive antibody Soon Paik
TaqMan Elise Palmer
Fluorometers Elise Palmer
Starch analysis Fay Papageorgiou
Request for Tobacco genomic Library Sudha Paramale
Tobacco Genomic Library Sudha Paramale
CAT Assay blues Tae-Ju Park
Primer & probe design software? Mick Partis
Protein dimerisation in SDS-gel Mick Partis
F9 cells & gelatinized plates Susan Patrick
Non-fat dry milk...really non-fat? Susan Patrick
Antibodies to His-tag Paul
Gla containing proteins in baculovirus John Pawlowski
ARGH! Northern Problems (again!) Aaron Pawlyk
Xenopus Pec
Rediprime labelling system Chip Peele
GFP mutant Chip Peele
Rapid Ligations Chip Peele
Rapid ligation Chip Peele
Taq-Man anyone? Pendragon
PCR from blood samples Pendragon
DNA in agarose gels Pendragon
Sequencing Kit required Pendragon
southern (discomfort) Pendragon
RNA extraction from small samples Pendragon
primer extension problems Daniel Pereira
How to 32P label a PCR probe? Daniel Pereira
DNAse treatment of isolated total RNA ? Daniel Pereira
Effect of LiCl on RT and enzymes Daniel Pereira
Boeringer-Manneheim cycle sequencing Daniel Pereira
PCR for leptospirose Lygia da Veiga Pereira
GFP mutant Stephen Perry
syber green Stephen Perry
------pure plasmid c LiCl---???? Pete
cutting totalDNA - a summary Petur Henry Petersen
Longe PCR problems. Petur Henry Petersen
southern (discomfort) Petur Henry Petersen
DNA stuck in agarose gel wells Petur Henry Petersen
primer extension problems Rolph Pfundt
DTT Stability Stephen P. Driska PhD
Cloning PCR products amplified using Us instead of Ts Dr Adrian Philbey
RDA of cDNA Dr Adrian Philbey
Cell culture question Dr Adrian Philbey
Help about Genereleaser Dr Adrian Philbey
HTLV and CTCL (was quote) Dr Adrian Philbey
fresh transformations for protein expression?? Andy Phillips
Phosphorescent Ink Andy Phillips
CMV-selectable marker A. Pischitzis
Vent gives blunt end products? (was: Re: ligation of blunt PCR products) Jan van der Ploeg
Non-radioactive primer extension Jan van der Ploeg
DNA precipitations David Plotner
frozen competent yeast protocol Scott Vande Pol
pT7-7 vector sequence Oliver Politz
strep vs spec Ian Polkinghorne
RT-PCR PROBLEMS Andrei Popov
coating pH? Andrei Popov
lysing yeast Andrei Popov
sequencing gels won't stick!! Doug Portman
Concealed Jennifer L. Potter
Western problems Jennifer L. Potter
Silver stain protocol C. S. Prakash
Meeting on Transgenic Plants: April 20-22, 1996 at Tuskegee Univ. C. S. Prakash
GUS antibody C. S. Prakash
WWW Medline Search? C. S. Prakash
In vitro protein phosphorylation William P. Prichett
half-life of phosphorimager screen William P. Prichett
GST-fusion protein purification William P. Prichett
quantification of total amino acids Colin Purrington
DNA in agarose gels QIAGEN
subscribe QUALICHEM at aol.com
SOE-PCR woes! Chanda Eva R
Requesting Technical Assistance REPLICATECH,INC.
help with research project related to antibodies RLH115
Lafmid BA vector RMCMAH93 at IRLEARN.UCD.IE
Adenylation site finding program? RMCMAH93 at IRLEARN.UCD.IE
T/A cloning HIRANYA ROYCHOWDHURY
T/A cloning again. To Kinase or Not HIRANYA ROYCHOWDHURY
Use a genomic clone to find the cDNA RZebrow134
subscribe Cherradi Rahmaoui
fresh transformations for protein expression?? Manoj Ramjee
Where to buy electrode wire Ahmed Rasheed
(no subject) Colin Rasmussen
In situ/ tubes Thermal Cycler? Jonathan Redfern
gene targeting Bernhard Redl
GFP in yeast Richard J. Reece
Yeast Colony PCR Richard J. Reece
DNA in agarose gels Van Reese
(none) Ted Reich
PCR-Ligation-PCR gene fusion protocol Mary P. Remington
CONTRACTORS - BOVINE MYELIN NEEDED Chris Remsperger
w=regel Essex Research
In situ/ tubes Thermal Cycler? John H Richburg
Protein Expression in E. coli S. Riley
Preps of PAC clones - help! Volkhard Rippe
what's an easy way to look for cell death? Robert W. St. G. Fisher, IV
Transgenic Handbook Robert. P McCabe, Jr.
Xsomal DNA Storage AGS. Robertson
(no subject) Inmaculada Garcia Robles
fresh transformations for protein expression?? Alberto I Roca
cell Death ELISA Felix Rockmann
THYMIDINE FREE MEDIA Elizabeth Rogers
GUS antibody Hilary Rogers
DNA precipitations Janet Rogers
growth of plasmid vectors in Stable 2 strain of E. coli Ronald E. Bishop, Ph.D.
growth of plasmid vectors in Stable 2 strain of E. coli Ronald E. Bishop, Ph.D.
Hybridisation Meshes Rory O'Brien, Biotechnology.
In Situ hyb. kit Wes Rose
Carafate, Sucralfate Wesley Rose
Wanted: Antibodies to Uroplakins Steffen Roth
DNA from agarose - home made spin columns? Hiranya Roychowdhury
SSCP and glycerol Heidi Ruijling
T7 transcribed RNA probes S.Bartoszewski
Carbon-dioxide independent medium S.K.Green
PCR from blood samples S.K.Green
RFLP standard protocol transformed plant cells? DOUGLAS SAKER
Promega minipreps crapping out JULES SILVERMAN
SSCP and glycerol Igor R Sagdeev
Chemical Mutagenesis of DNA Jerry Salem
Clone Manager Klaus Salger
Is this newsgroup archived? Klaus Salger
argU plasmid Maria Santos
In situ/ tubes Thermal Cycler? Jasper Saris
Looking for Avidin Beads Y. Sato
Protein dimerisation in SDS-gel Lauge Schaffer
Help please: immunolabelling with 2 antibodies from same animal David A. Schiffmann
Hela with T7 RNA-polymerase Martin Schindler
TC-100 Denni Schnapp
Locate Kikuya Kato? Dr. J.P. Schofield
trafo of bacillus stearothermophilus Holger Scholz
Troubles with Pfu polymerase Holger Scholz
How to transfer plasmids???? Edgar Schreiber
Know company? Dr. Ulrich Schroeder
lexA antibodies searched Frank Schulte
CG-Repeats in Cycle Sequencing Axel Schumacher
Warning! More Membrane SWAPs Axel Schumacher
superbroth Ralph Schuster
subscribe Tanja Schwuchow
How many restriction sites fragments per genome? Geoff Scopes
fresh transformations for protein expression?? R.K. Scopes
where to get antibody 9E10? Bart Sefton
CAT Assay blues Palle Serup
superbroth Robert Seymour
Diff. Display Info. Robert Seymour
soluble protein expression in E. coli Robert Seymour
Need info re DNA-spec. dyes Hr Dr. S. Shapiro
Test questions for molec. biol? Glen Shearer
Thermostable reverse transcriptase Kathleen Shelton
RNAse for phage preps Juqun Shen
Question on how to radio-label a protein. Dana Shima
Ab for poly(ADP-ribose)polymerase. Incheol Shin
Quick RNA prep methods needed Mari L. Shinohara
PCR Amplification of Herpes from Infected Cells D.A. Shivak
Blocking Phosphotyrosine Blots David Shock
PCR from whole blood (PE/ABI GeneScan) Benny Shomer
Protocol for Re-using of Qiagen tips Song-Kun Shyue
5'oligolabelling kit ? Hiljar Sibul
PAAG polymerization problem Hiljar Sibul
Sequenase Hiljar Sibul
Looking for shuttle vector S.sanguis - E.coli Igor Sidorenkov
Troubles with Pfu polymerase Nathan Siemers
GFP in yeast Sandy Silverman
PCR-Ligation-PCR gene fusion protocol Sjohnson at biosci.mbp.missouri.edu
Glutathione-HRP conjugate Robert Slany
LI-COR distributor in UK? H.J. Sluiman
Troubles with Pfu polymerase Koen De Smet
What do you think? Koen De Smet
soluble protein expression in E. coli Koen De Smet
SDS-PAGE:slower migration after reducing? John Edward Smith
Non-fat dry milk...really non-fat? Mark Solomon
X-ray film developers Keld Sorensen
monovalent streptavidin Keld Sorensen
Western problems Keld Sorensen
Phosphorescent Ink Keld Sorensen
Looking for Avidin Beads Keld Sorensen
Coating-pH ? Keld Sorensen
Fluorescence-labelled biotin Keld Sorensen
Glass wells needed Keld Sorensen
Immunoprecipitation's theory Giorgio Spagnol
Redundancy in kinased protein detection Giorgio Spagnol
pH of TE Giorgio Spagnol
Help with PCR reference Giorgio Spagnol
Different behaviour of a glycoprotein in one dimension and 2D blots. Giorgio Spagnol
(no subject) Giorgio Spagnol
PCR directed mutagenesis. Primer design Giorgio Spagnol
Desperately seeking Giorgio Spagnol
help: c-fos probe Cathy Sprankle
Plasmid drawing software Christopher Spry
Plasmid drawing software Christopher Spry
Sequencing mRNA from variable regions Anne Spurkland
RDA of cDNA Peter Staeheli
RDA of cDNA Peter Staeheli
SDS interference in molecular weight determination?? Marc Stees
SDS interference in molecular weight determination?? Marc Stees
Concealed (Genequant II) Steve Stelman
fresh transformations for protein expression?? Kaj Stenberg
Is Insect 28 and 18S banding different ? Edwin Stephenson
The "megaprimer" method of PCR Steve
FW: Small basic proteins Stewart, Robert
Rhodococcus phage Q4 Matthew Stoecker
Silver stain protocol Andreas M. Stolpe
HELP: biotin-ATP source wanted Steven Sullivan
rt-pcr problems William Sun
genomics with SalI or AatII? Dr.Sailesh Surapureddi
Carrier DNA inhibits transfection Dr.Sailesh Surapureddi
Protein interaction studies - co-expression in bacteria? Dr.Sailesh Surapureddi
request for pBS vector DNA sequence Dr.Sailesh Surapureddi
Protein dimerisation in SDS-gel Dr.Sailesh Surapureddi
Lysing Yeast Dr.Sailesh Surapureddi
Why is it _glacial_ acetic acid??? and NaAc question Dr.Sailesh Surapureddi
DNA stuck in agarose gel wells Dr.Sailesh Surapureddi
Hydroxyapatite, how does it work? Dr.Sailesh Surapureddi
sequence analysis packages? Claudia Sutton
fresh transformations for protein expression?? Michael Szardenings
ligation of blunt PCR products Michael Szardenings
transformation efficiency of amplified plasmids Michael Szardenings
prep of DNA from Biolabs' vent-pol 10x buffer? Michael Szardenings
Phosphorescent Ink A.P.R.M. OSTEROP at TCH
PCR from whole blood (PE/ABI GeneScan) A.P.R.M. OSTEROP at TCH
PCR using Us instead of Ts A.P.R.M. OSTEROP at TCH
Anti-Mycoplasma agents A.P.R.M. OSTEROP at TCH
College Experiment THORN at LAN.TJHSST.EDU
T/A cloning Simon TWigger
transgenic mouse cost!!!!! Jil Tardiff
SDS-PAGE:slower migration after reducing? Ron Tate
DTT Stability Ron Tate
removing nucleic acids from coli extracts? Ron Tate
TaqStart hot-start reagent Dr G R Taylor
RDA and polymorphism Tedibear
TriZol results? Tedibear
Low temperature freezer Dennis Templeton
VENTREX Labs, Portland, Maine Tom Thatcher
Looking for HYCOR or VENTREX Tom Thatcher
Removing DNA from Slot Blotter Tom Thatcher
Removing DNA from Slot Blotter Tom Thatcher
Need rat TNF-R Antibody Brian S. Thoma
Need rat TNF-R antibody Brian S. Thoma
kinetics of ITP incorperation by RNA polymerases Matt Thomas
Question: lambda plaques not transparent, bacterial lawn not , opaque Michael Thompson
Thermostable reverse transcriptase Jan Ulrik Thorup
--> CALL FOR PAPERS <-- Dietmar Tietz
Problems with RT-PCR Anthony Tomlinson
UK Supplier of BIO 101 products Anthony Tomlinson
Is linear DNA mandatory for PCR amplification ?? Anthony Tomlinson
T/A cloning again. To Kinase or Not Anthony Tomlinson
Antisense and sensibilities Joanne Tornow
mtDNA probe templates anyone??? Alfred Trautweiler
Why is it _glacial_ acetic acid??? and NaAc question Ken Trimm
transfection HL-60 Bruce R. Troen
housekeeping gene rtpcr Anthony Trombino
Q: Separation of 12bp ds-oligo from ss Vadim Tsvetnitsky
Regeneration of Ni-NTA agarose Vadim Tsvetnitsky
How much for IPs?! Michael J. Tuvim
Yeast Colony PCR Simon Twigger
Sequence of pAS1 is available Curtis Tyree
News for pAS1 two hybrid users Curtis Tyree
Tailed-Template Assay? UCI
Help: Ligation of ss Oligo to 5' overhang UCI
Plant genomic DNA extraction protocol? Paul E. Ulanch
Subcloning large PCR products Paul E. Ulanch
Low Fidelity PCR Mutagensis ??s Tim Uphoff
Slot Blot limitations Jeffrey Upperman
Sequencing query Virginia Urquidi
Plasmid copy number determination Edgar Valencia
DNA precipitations Edgar Valencia
Automatic DNA sequencing: what's the best? Claudio Valsangiacomo
Troubles with Pfu polymerase Margon Vandongen
PCR polymerases Margon Vandongen
Promega minipreps crapping out Margon Vandongen
Help - FESMID. Dominique Vanhee
northerns - sharp bands Tom Vasicek
northerns - sharp bands Tom Vasicek
northern transfer efficiency Tom Vasicek
Actin mRNA sizes and alpha-beta %identity Tom Vasicek
Li-Cor vs ABI Sequencers Tom Vasicek
Non-fat dry milk...really non-fat? Tom Vasicek
Northern Problems - Disolve RNA in FORMAMIDE Tom Vasicek
northerns - sharp bands - SOLUTION, THANKS!! Tom Vasicek
Need a rapid method to check transgenicity in mice? in mice Tom Vasicek
Phosphoimager Tom Vasicek
sequencing gels won't stick!! Tom Vasicek
Looking for antibodies against Cre-recombinase Mark Veugelers
Looking for antibodies against Cre-recombinase Mark Veugelers
Looking for antibodies against Cre-recombinase Mark Veugelers
Sense/Antisense nonsense Mr. I.S. Viney
Quickest method to visualize plasmid presence in bugs? Mr. I.S. Viney
Silver stain protocol Marc Visconti
Enzyme Mismatch Cleavage NUMI Visitor
Anti-Mycoplasma agents Matthias Vogel
Help on cDNA library construction needed Killerhippie Volker
Need help in detecting OCT-1 Amy Voltz
SSCP Dominic Voon
Looking for Avidin Beads Michael Vork
help for QIAGEN (QIAprep Spin) Vozier
Vent gives blunt end products? (was: Re: ligation of blunt PCR products) Stephane Vuilleumier
T/A cloning W.Coco
T/A cloning W.Coco
Chelex-100 vs. GeneReleaser? W.Coco
PCR from PAA gels? W.Coco
magnetic bead losses during manipulation W.Coco
why not 2x TAE instead of TBE? W.Coco
upper limit to Inverse PCR products? W.Coco
Lambda Exonuclease and Sequencing PCR Products W.Coco
PCR from blood samples W.Coco
magnetic bead losses during manipulation W.Coco
magnetic bead losses during manipulation W.Coco
5'oligolabelling kit ? Toshihiro WATANABE
Antibodies to His tag. Tim Wachtmann
rt-pcr problems Belinda J Wagner
DNA from agarose - home made spin columns? Mark Walberg
Ethanol precipitations: disposable 30 ml tubes? Mark Walberg
Promega Magic David W Walker
hns-E.coli Kimberly Walker
upper limit for transformation Edward Wang
Expression of fusion proteins w/ GIII Peter Wang
In-Vitro Mutagenesis of BACs, PACs or Cosmids - any Suggestions ? Peter Wang
directed heteroduplex analysis experiences? Peter Wang
the most outrageous ripoff Weigwang Wang
perplexing cloning difficulties Bill Warren
Preps of PAC clones - help! Dr Paul Watt
Transfection/Transgenics: protocols for BIG DNA Fragments Dr Paul Watt
resuspending intractable DNA CK Wen
resuspending intractable DNA CK Wen
differential display CK Wen
RNA isolation and cDNA synthesis CK Wen
mRNA isolation problem CK Wen
Diff. Display Info. CK Wen
How to 32P label a PCR probe? CK Wen
PCR from single colonies? CK Wen
Temperatures for precipitation of DNA CK Wen
Help: potato DNA isolation CK Wen
Tiny amount mRNA CK Wen
Help on cDNA library construction needed CK Wen
Help on cDNA library construction needed CK Wen
q: stability of 32p-RNA CK Wen
southern (discomfort) CK Wen
DNA stuck in agarose gel wells CK Wen
Protein pI and migration on an SDS-PAGE gel CK Wen
DNA precipitations CK Wen
Shortened products with pET vector CK Wen
Ethanol precipitations: disposable 30 ml tubes? CK Wen
Lambda Screening Problems CK Wen
DNA precipitation CK Wen
RNA extraction from < 50 mg Chi-Kuang Wen
probs digesting plant genomic Chi-Kuang Wen
RNAse without DNAse (low salt)? Chi-Kuang Wen
Hydroxyapatite, how does it work? Chi-Kuang Wen
DNA from Sorbus,HELP! Jon West
probs digesting plant genomic Jon West
Re glass wells needed Lesley Weston
Antibodies to His tag. Weswillwin
Longe PCR problems. Don Wigington
DNase II freezing? William B. Melchior, Jr.
More and more confused about making SENSE Erik A. Williams
Neuronal cell culture Mr D.J.A. Wilson
Course Announcement Rick Wilson
Does His-tag have to be terminal? Troels Wind
Alternatives to amber codon? Troels Wind
ras, raf-1, GAP clones wanted Troels Wind
sequencing gels won't stick!! Kimberly Ann Winkeler
DNA fixation on Agarose gel Harry J. Witchel
southern (discomfort) Harry J. Witchel
DNA stuck in agarose gel wells Harry J. Witchel
Sense-Antisense, genuine confusion Harry J. Witchel
Question: lambda plaques not transparent, bacterial lawn not opaque Harry J. Witchel
Ethanol precipitations: disposable 30 ml tubes? Harry J. Witchel
Total RNA from blood Stephan Witte
Help! total RNA from blood/leukocytes? Stephan Witte
50-80 bp size marker Arthur Wohlwill
Terrific Broth Lisa Wohnlich
ras, raf-1, GAP clones wanted Jim Woodgett
Antisense and sensibilities Marci Wright
Promega minipreps crapping out Marci Wright
superbroth Marci Wright
help!! What do you do when PCR not work? Zhengyang Wu
Antisense and sensibilities NARENDRA S YADAV
directional cDNA cloning Mayumi Yagi
Phosphorescent Ink Mayumi Yagi
Date:18 Janunery 1996 Yu Yanxing
(none) Yu Yanxing
scFv help Yu Yanxing
Sequencing from phage DNA Ann M. Yezerski
seq. lambda gt22 Ann M. Yezerski
WWW Medline Search? Ian A. York
New website: Combinatorial mutagenesis, Imaging, GFP, etc. Youvan
exon trapping etc David Tak Yan Yu
mRNA induced by bacteria invasion David Tak Yan Yu
expression of cloned E. coli csp David Tak Yan Yu
RNA extraction from < 50 mg Paquette Yves
grant & funding newsgroups/websites? Chaoyang Zeng
CapFinder PCR cDNA Library Construction Kit Frances Hannan Zoology
northerns - sharp bands Frances Hannan Zoology
HPLC systems aandonov at HPB.HWC.CA
Looking for an address aandonov at HPB.HWC.CA
Sirius Red for Collagen Quant? aelston
Sirius Red for Collagen Quant.? aelston
RNA extraction ahung at ASRR.ARSUSDA.GOV
RNA extraction kit ahung at ASRR.ARSUSDA.GOV
Static electricity and pipettes alamarch
T/A cloning alamarch
Please, help me alamarch
DNA extraction and PCR from Ethanol preserved samples alamarch
Tracking dyes in agarose gel electorphoresis alamarch
Sirius Red for Collagen Quant? alenkd at nwrain.com
THYMIDINE FREE MEDIA an456680 at anon.penet.fi
T/A cloning eric anderson
soluble protein expression in E.coli dorina avram
Pharmacia e-mail address dorina avram
SSCP bagley at FAUNA.UCDAVIS.EDU
syber green baharvey at swansea.ac.uk
PCR from whole blood (PE/ABI GeneScan) bb1dopeg
Cheap glass plates for ABI automated sequencer bb1dopeg
adress of GENOME SYSTEMS bca11 at oc3gra.org.chemie.tu-muenchen.de
Troubles with Pfu polymerase benedik at uh.edu
PCR from PAA gels? bergwitz at helix.mgh.harvard.edu
PEST score ducommun bernard
PEST score ducommun bernard
FRENCH DNA sequencing service ducommun bernard
gene targeting bernhard.redl at uibk.ac.at
HELP! How do you purify a 150bp PCR product for sequencing berns001 at coyote.csusm.edu
Methods of isolating RNA and DNA betts at ORION.ETSU.EDU
RNAse for phage preps WWW-Brower auf bigapple/1.Stock
RNAse for phage preps WWW-Brower auf bigapple/1.Stock
UV-VIS difference spectroscopy javier blanco
test javier blanco
test from cba javier blanco
In Situ hyb. kit boyce at cshl.org
Transforming ssDNA into JM 109 boyce at cshl.org
Antisense and sensibilities john brennand
PCR library using M13 and specific internal primer john brennand
RACE john brennand
CONTRACTORS - BOVINE MYELIN NEEDED john brennand
Sequencing from phage DNA john brennand
PCR and DMSO john brennand
NeoR alternative vectors? john brennand
RNAse for phage preps john brennand
RNAse for phage preps john brennand
Cacodylate & CoCl2 john brennand
HPRT john brennand
How many restriction sites fragments per genome? john brennand
Carbon-dioxide independent medium john brennand
soluble protein expression in E. coli john brennand
Is linear DNA mandatory for PCR amplification ?? john brennand
Does Ultrasound destroy dsDNA? john brennand
Non-fat dry milk...really non-fat? john brennand
Antibodies to His tag. john brennand
Inexpensive Balance john brennand
Temperatures for precipitation of DNA john brennand
EcoB supplier ? john brennand
EcoB supplier ? john brennand
Commercial pre-made Notherns john brennand
Agar replacement john brennand
Ethanol precipitations: disposable 30 ml tubes? john brennand
eppendorf tube closures john brennand
Agar replacement brett
Ethanol precipitations: disposable 30 ml tubes? brett
Plasmid rescue from S.cerevisiae paul bundock
(no subject) c.b.ko
Preparation of Androgen for tissue culture c.b.ko
ligation of blunt PCR products calaniz
to Van Reese and Webbers carlo at florina.angers.inra.fr
Antisense and sensibilities chihara
what's an easy way to look for cell death? steven joseph chmura
Phosphorescent Ink daj at nhm.ac.uk
Anyone doing Maxam-Gilbert chemistry?-help! damania
superbroth davesmith at bioch.tamu.edu
His-tagged proteins; Ni-NTA; contamination davesmith at bioch.tamu.edu
superbroth davesmith at bioch.tamu.edu
fresh transformations for protein expression?? davesmith at bioch.tamu.edu
Quantification of Westerns davesmith at bioch.tamu.edu
Antibodies to His tag. davesmith at bioch.tamu.edu
Does His-tag have to be terminal? davesmith at bioch.tamu.edu
Small basic proteins davesmith at bioch.tamu.edu
Protein dimerisation in SDS-gel davesmith at bioch.tamu.edu
LI-COR distributor in UK? simon day
amplified inserts dbjerm at foothill.net
What is pRK7 vector? delabess
reques for used equipment dessybd at iph.bio.acad.bg.
tilted gels - gel.log (0/0) dtn
tilted gels - gel.log (1/0) dtn
tilted gels - gel.log (0/0) dtn
PCR of cDNA insert in pUC 18 dummy
Thermostable reverse transcriptase dummy
Conference fang at SASK.USASK.CA
Nuclear Runoff Assay farrell at dnatech.com
reverse transcriptase fill_in_your_full_name
reverse transcriptase fill_in_your_full_name
Help! total RNA from blood/leukocytes? fill_in_your_full_name
Diatom/Celite fill_in_your_full_name
Antibodies to His tag. g830204 at ym.edu.tw
Any CsCl pure method helpful hints? gedmunds at kean.ucs.mun.ca
------pure plasmid c LiCl---???? gedmunds at kean.ucs.mun.ca
PFGE trouble>Hi there, > My problem with my PFGE apparatus is I am getting streaks instead of bands. I believe that this is related to the fact that my buffer is giving slightly lower mA readings (18 instead of 21; 15 instead of 18) than usual. I have re-made the buffer exactly the same way that I always do when I get high quality results. I sent the power source in for repair, as I did 6 months ago when the same thing happened, and they found the the readings were slightly HIGH and re-calibrated the power supply. Since I got the power supply back, I have run a 24 hour gel with standards in 0.5 X TBE that came out OK. The normal gel that I am running, with 10 times less EDTA hence, 0.5 X TBe, has resulted in a continuous smear. >I also ran a PFGE gel on another machine, in another lab, with THE SAME buffer from the THE SAME bottle, and it came out well. Thank you, in advance, for your help. nkh@sage.purdue.edu Dunkles group
PFGE trouble>Hi there, > My problem with my PFGE apparatus is I am getting streaks instead of bands. I believe that this is related to the fact that my buffer is giving slightly lower mA readings (18 instead of 21; 15 instead of 18) than usual. I have re-made the buffer exactly the same way that I always do when I get high quality results. I sent the power source in for repair, as I did 6 months ago when the same thing happened, and they found the the readings were slightly HIGH and re-calibrated the power supply. Since I got the power supply back, I have run a 24 hour gel with standards in 0.5 X TBE that came out OK. The normal gel that I am running, with 10 times less EDTA hence, 0.5 X TBe, has resulted in a continuous smear. >I also ran a PFGE gel on another machine, in another lab, with THE SAME buffer from the THE SAME bottle, and it came out well. Thank you, in advance, for your help. nkh@sage.purdue.edu Dunkles group
Teflon-coated slides in Australia h.woo
Email address of R & D of Amersham, UK h.woo
DNA from Sorbus, HELP! paul hand
subscribe chris harris
EtOH precipitation temp jim hartley
DNA solution or suspension? jim hartley
Troubles with Pfu polymerase Yves hatzfeld
primer extension problems helix.nih.gov
housekeeping gene rtpcr helix.nih.gov
commercial suppliers of slides for in situ hybridization james anthony holzwarth
commercial suppliers of slides for in situ hybridization 2 james anthony holzwarth
Research Assistant wanted (PhD student) hschief at gwdg.de
cloning of repetitive DNA hshibat at ibm.net
fresh transformations for protein expression?? ijiwaru at wheel.dcn.davis.ca.us
ABI Sequencer problem j.r.sayers
Cured ABI373 j.r.sayers
Multiple bands on DGGE j.r.stephen
SSCP and glycerol j.r.stephen
BsaBI digests jlight at RESUNIX.RI.SICKKIDS.ON.CA
Rediprime labelling system joaroyo at samba.cnb.uam.es
College DNA Experiments judi_heitz at stratagene.com
Nuclear Runoff Assay k
Tetracycline-free bovine serum k
Lafmid BA vector??? k.d.preuss
JRH Bioscience in Europe ???? k.d.preuss
Promoter elements Database???? kalch
REPOST: Help with Somatic cell hybrids mouse x human kanemats at morgan.angis.su.oz.au‚
Help: RNA preparation: very low yields with spinal cord; pH influence? kang at msvax.mssm.edu
Promega Magic kang at msvax.mssm.edu
Commercial pre-made Notherns kang at msvax.mssm.edu
Promega minipreps crapping out kang at msvax.mssm.edu
northerns - sharp bands - SOLUTION, THANKS!! kang at msvax.mssm.edu
PCR polymerases alan kosaka
S1 nuclease seq pref? kvernick at helix.nih.gov
Finding concentrations of polysaccharides l14y at unb.ca
anti-Bcl-2 antibody lasgo.bbs at AIDEBBS.EDU.TW
Quantify mRNA in PCR lcwilliams at medusa.unm.edu
PCR on human tissues lcwilliams at medusa.unm.edu
hot start beads? lees at SASK.USASK.CA
nitroso-guanidine (NG) protocol? lees at SASK.USASK.CA
pBS vector sequence liz_caetta at stratagene.com
In vitro transcription lmjakt at hkucc.hku.hk
mRNA prep. by magnetic beads logan
SDS-PAGE:slower migration after reducing? logan
sequencing gels won't stick!! logan
RT-PCR quantification controls logan at MSDOS.ENSAM.INRA.FR
PCR fidelity m.a.jones
capped or not capped RNA transcript? maken
capped or not capped RNA transcript?(correction) maken
SKUMIX paolo de marco
DNA in agarose gels Rolf marteijn
T/A cloning again. To Kinase or Not glover martin
differential display kit ???? matthias
TA-cloning patented? mc97 at musica.McGill.CA
How to 32P label a PCR probe? mikem at s-and-s.com
rnase free pipet tips mobio at electriciti.com
extractin mtDNA from fish scales mobo at mc.edu
Biolog nobody at nowhere
pBS vector sequence nsaunders at molbiol.ox.ac.uk
PCR using Us instead of Ts nswema05 at angis.su.OZ.AU
Temperatures for precipitation of DNA nswema05 at angis.su.OZ.AU
PCR from human tissues nswema05 at angis.su.OZ.AU
TAQSTART HOT-START REAGENT nswema05 at angis.su.OZ.AU
lactometer oRutebuka
oligonucleotide probe design patricia ohana
fresh transformations for protein expression?? ohannes1 at jeflin.tju.edu
P1 cloning: HELP otello at sc2a.unige.ch
Inexpensive Balance pagg
Diff. Display Info. perkitm at WKUVX1.WKU.EDU
Antisense and sensibilities - the real story pgegen at rnaworld.bio.ukans.edu
Phosphorescent Ink - Sources pgegen at rnaworld.bio.ukans.edu
Phosphorescent Ink - Sources pgegen at rnaworld.bio.ukans.edu
SOE PCR woes pskaytes at INTNET.UPJ.COM
UK Supplier of BIO 101 Products ptan
Electroporation of mammalian cells Christian reiser
"revolutionary" subtractive hybridization... rhubner at molbiol.ox.ac.uk
Tissue Culture and mucin robiso at vt.edu
tissue explants robiso at vt.edu
tight junction proteins robiso at vt.edu
Subcloning large PCR products rosasg01 at mcrcr6.med.nyu.edu
differential display kit ???? rover at livnet.com
Problems with RT-PCR rover at livnet.com
DNA purification from yeast rr
Sequencing problem s060965 at AIX1.UOTTAWA.CA
Transgenic Handbook s060965 at AIX1.UOTTAWA.CA
cutting totalDNA - a summary s060965 at AIX1.UOTTAWA.CA
Commercial pre-made Notherns sunil shaw
Would you use high thruput sequencer? shohle at ibm.net
removing nucleic acids from coli extracts? trey simmons
Promega minipreps crapping out trey simmons
How to 32P label a PCR probe? spolsky at say.acnatsci.org
Stubborn DNA during resuspension spyong at pc.jaring.my
Effect of LiCl on RT and enzymes spyong at pc.jaring.my
Oligotex from Qiagen vs column spyong at pc.jaring.my
RNAse Inhibitor stefan.harjes at rz.ruhr-uni-bochum.de
RT-PCR PROBLEMS suihuang at usa1.com
DNA isolation from MUSHROOMS. Need help. suihuang at usa1.com
fluorescence PCR suihuang at usa1.com
What do you think? syamada at sb.gunma-u.ac.jp
Preps of PAC clones - help! user at ichr.uwa.edu.au
Preps of PAC clones - help! user at ichr.uwa.edu.au
fixation-sensitive antibody username at bay.cc.kcl.ac.uk
Promega minipreps crapping out varyc.mmcri at mmc.org
Agar replacement verlooy at uia.ua.ac.be
Quantification of Westerns gerard wall
Help for M9 plate fei wang
directional cDNA cloning Ron Goto x2832
IEF/IMMUNOBLOTTING, help Ron Goto x2832
pGEX system meller yael
PCR and DMSO ynakao at kean.ucs.mun.ca
SSCP ynakao at kean.ucs.mun.ca
SSCP and glycerol ynakao at kean.ucs.mun.ca
How to transfer plasmids???? ynakao at kean.ucs.mun.ca
Chemical Mutagenesis of DNA you at somehost.somedomainGARETHJENKINS
pH of TE you at somehost.somedomainGarethJenkins
Mouse tissue turnover you at somehost.somedomainGarethJenkins
DNA Sequencing Services xiu yu
non-adhesion materials xiaoke zhang
extract RNA from peritoneal macrophages xiaoke zhang
teflon plates xiaoke zhang
Sensitivity of Luciferase Assay zhangxia at post.queensu.ca
codon usage zinc

Last message date: Wed Jan 31 23:29:05 EST 1996
Archived on: Sun Mar 6 20:55:05 EST 2005

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