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Antibody against phosphoserine/threonine

Jim Woodgett jwoodget at oci.utoronto.ca
Mon Jul 1 13:08:53 EST 1996

In article <31D7ED66.3535 at ens-lyon.fr>, Stephane Ory <Stephane.Ory at ens-
lyon.fr> writes:
> Does anyone know where antibody against phosphoserine and 
> phosphothreonine are available ? 
> Thanks per advance 

Monoclonals are available from Sigma but when we tested them, they were not 
useful.  Phosphoserine and phosphothreonine are about 100-1000X more abundant 
in proteins that phosphotyrosine so you need an additional level of 
specificity.  A number of companies are selling antibodies to proteins that 
only detect those proteins if a particular ser/thr/tyr residue is 
phosphorylated.  See NEB and UBI catalogues for example.

Examples of phospho-specific antibodies to the following proteins:

tau (microtubule-associated protein, various ser/thr)
Jun (ser 63)
STAT 3 (tyr 705)
ERK1/2 (tyr 204)
p38 MAPK
CREB (ser 133)

Jim Woodgett

Ontario Cancer Institute     mailto:jwoodget at oci.utoronto.ca
610 University Avenue       http://kinase.oci.utoronto.ca/woodgettlab.html
Toronto, Ontario M5G 2M9   phone (416) 946-2962  fax: (416) 946-2963

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