In article <31D7ED66.3535 at ens-lyon.fr>, Stephane Ory <Stephane.Ory at ens-
lyon.fr> writes:
> Does anyone know where antibody against phosphoserine and
> phosphothreonine are available ?
>> Thanks per advance
Monoclonals are available from Sigma but when we tested them, they were not
useful. Phosphoserine and phosphothreonine are about 100-1000X more abundant
in proteins that phosphotyrosine so you need an additional level of
specificity. A number of companies are selling antibodies to proteins that
only detect those proteins if a particular ser/thr/tyr residue is
phosphorylated. See NEB and UBI catalogues for example.
Examples of phospho-specific antibodies to the following proteins:
tau (microtubule-associated protein, various ser/thr)
Jun (ser 63)
STAT 3 (tyr 705)
SEK1
ERK1/2 (tyr 204)
p38 MAPK
CREB (ser 133)
Jim Woodgett
Ontario Cancer Institute mailto:jwoodget at oci.utoronto.ca
610 University Avenue http://kinase.oci.utoronto.ca/woodgettlab.html
Toronto, Ontario M5G 2M9 phone (416) 946-2962 fax: (416) 946-2963
