in situs with oligos?
S.K.Green at shef.ac.uk
Mon Jul 1 04:54:14 EST 1996
> We are doing in situ hybridization on sections (paraffin, 35S,
> emulsion,.......we were thinking of synthesizing oligos and labelling
> them for in situs. Is this possible? Has anyone tried it and gotten
> it to work?
No problem, our lab routinely uses oligos for in situ. The oligos are
usually 30-60mers. We 3' end label/tail (using terminal transferase and
35 S). If you need any further info. let me know and I will dig out a
reference and send you the protocol.
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