in situs with oligos?

S.K.Green S.K.Green at shef.ac.uk
Mon Jul 1 04:54:14 EST 1996


> We are doing in situ hybridization on sections (paraffin, 35S, 
> emulsion,.......we were thinking of synthesizing oligos and labelling 
> them for in situs.  Is this possible? Has anyone tried it and gotten
> it to work?
> 
> Thanks,
> --Julia


Julia,

No problem, our lab routinely uses oligos for in situ.  The oligos are 
usually 30-60mers.  We 3' end label/tail (using terminal transferase and 
35 S).  If you need any further info. let me know and I will dig out a 
reference and send you the protocol.

Sue



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