method for extracting DNA and RNA from same sample.

Jean-Francois Larrivee jfl at rsvs.ulaval.ca
Wed Jul 3 16:01:57 EST 1996


On 3 Jul 1996 15:58:13 GMT, Terese Maltzman <maltzman at essex.UCHSC.edu>
wrote:

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>Does anyone have a failproof method for extracting RNA and DNA from the 
>same tissue (in this case it is a tiny mouse tumor) so that RT-PCR can 
>be done with the isolated RNA and PCR can be done with the isolated DNA?

Try TRIZol reagent from Gibco-BRL, it works fine for RNA isolation and
the protocol includes a DNA isolation from the same sample.

Jean-Francois





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