Transfection of pulmonary artery smooth muscle cells with an adenovirus vector

[mahayden at med.unc.edu]

We are having trouble tranfecting our rat pulmonary artery smooth muscle 
cell line and RAW transformed mouse macrophage line with our adenovirus 
vector. The vector has worked in 3T3 cells, CF-21 human airway epithelial 
cells, and MVILU mink epithelial cells but the smooth muscle cells 
and RAW cells seem to be resistant to transfection. I was wondering if 
anyone has been successful with the transfection of smooth muscle cells 
with viral vectors, or if anyone has any suggestions that may lead to 
improved transfection with smooth muscle or macrophage cells.