malexeyev at biost1.thi.tmc.edu
Thu Jul 4 15:26:32 EST 1996
In article <nico.13.004426D7 at lmb1.rug.ac.be>, nico at lmb1.rug.ac.be wrote:
> In article <31CA186E.61AE at uci.edu> bparekh at UCI.EDU (Wes Hatfield) writes:
> >Does anyone know how long rifampicin is goof for once it is preapared in
> >ethanol? In my hands it appears to loose activity in a couple of days,
> >as judged by in vitro transcription assays. Thank you
> Thats right, rifampicin is best made up fresh just before use, in ethanol or
I used to prepare 3-5ml of 50 mg/ml rifampicin in DMSO and used it for at
least couple of month untill it is being used up. Stores at +4C.
I must mention that I used it for selection of resistant E. Coli at 50-100
Hope this helps.
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