Gene Sequencing

Sandra Pena De Ortiz spenadeo at
Thu Jul 4 10:49:15 EST 1996


I am screening a genomic library and have been considering several 
approaches to sequence the gene I am looking for.  I have 1.5 kb of cDNA 
sequence.  I am considering shot gun sequencing, restriction mapping and 
then sequencing, or primer walking.  Does anyone have comments or 
suggestions?  They will be greatly appreciated.


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