SmaI digest

Steven Tait stait at lab0.vet.ed.ac.uk
Thu Jul 4 07:55:24 EST 1996


bimvh at zeus.bris.ac.uk (MV. Hayes) wrote:
>
> The Boeringer catalogue says to digest at 25C but I didn't get any 
> digestion after 4hrs so I would like to know what temperature does everyone 
> else use with SmaI?

Some say 25C some say 30C - both should work if you follow the
conditions specified. Did you try a control sample that you know
digests OK? Most likely the enzyme or buffer is off.
Steven




More information about the Methods mailing list