stait at lab0.vet.ed.ac.uk
Thu Jul 4 07:55:24 EST 1996
bimvh at zeus.bris.ac.uk (MV. Hayes) wrote:
> The Boeringer catalogue says to digest at 25C but I didn't get any
> digestion after 4hrs so I would like to know what temperature does everyone
> else use with SmaI?
Some say 25C some say 30C - both should work if you follow the
conditions specified. Did you try a control sample that you know
digests OK? Most likely the enzyme or buffer is off.
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