RT-PCR Problems

S K Green S.K.Green at shef.ac.uk
Thu Jul 4 05:31:08 EST 1996

Paul Mozdziak wrote:
> I've solved my problem with multiple PCR Bands--BUT now I've got one
> band at a larger number of bp than I should have--any ideas?

Paul, do you have any idea what size your genomic product may be?  If 
your RT-PCR primers span exons, then you may be getting the genomic 
product which should be larger. Or, maybe you have a different splice 
product (which is larger).  Do you get the expected size band at all, or 
do you always get the larger product?

Hope that helps,

Sue Green, PhD

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