S K Green
S.K.Green at shef.ac.uk
Thu Jul 4 05:31:08 EST 1996
Paul Mozdziak wrote:
> I've solved my problem with multiple PCR Bands--BUT now I've got one
> band at a larger number of bp than I should have--any ideas?
Paul, do you have any idea what size your genomic product may be? If
your RT-PCR primers span exons, then you may be getting the genomic
product which should be larger. Or, maybe you have a different splice
product (which is larger). Do you get the expected size band at all, or
do you always get the larger product?
Hope that helps,
Sue Green, PhD
More information about the Methods