Subtractive cDNA

Veronique BOYER boyer at lyon151.inserm.fr
Thu Jul 4 06:06:58 EST 1996


Hi. I checked in the Clontech catalog, and if you are talking about the
Delta RNA fingerprinting, I would not use this kit. I work with the
Differential Display (or RAP-PCR) since 2 years and I got most of the time
false positive. I would recommend now to use the approach published by
Sokolov and Prockop, Nucl. Acid Res.,1994, 22:4009, which is still
arbitrary PCR, but with a lot of advantages.



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