Elisa for Q-RT-PCR

Franz Weilbach neuk138 at rzbox.uni-wuerzburg.de
Thu Jul 4 16:12:07 EST 1996


to avoid handling with radioactive dNTPs, we cuurently plan to establish 
a ELISA method for mRNA determination. A biotinylated capture primer 
attaches the PCR product to a streptavidin coated microplate. The 
captured DNA is then detected after annealing wiht DIG labeled primers.

Has anybody experiences with this method? Any recommendations would be 
appreciated. 

Thanks
Franz Weilbach
Neurol Dept Uni Wuerzburg




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