Cohesive End Ligations - Question

Kathy L kathryn at U.Arizona.EDU
Fri Jul 5 18:04:02 EST 1996


On Tue, 2 Jul 1996, Kathy L wrote:

> Is it possible to run cohesive end ligations too long - i.e. would you 
> end up with high-molecular weight concatomers?  I'm getting some 
> high-weight stuff when I run a gel and the transformations aren't working.
> 

Thanks to everyone who has helped me out with this.  It appears my 
problem was too much DNA in the reaction -- I dropped the concentration 
from ~1 ug to 80 ng and it worked wonderfully.

Kathy




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