Cohesive End Ligations - Question
kathryn at U.Arizona.EDU
Fri Jul 5 18:04:02 EST 1996
On Tue, 2 Jul 1996, Kathy L wrote:
> Is it possible to run cohesive end ligations too long - i.e. would you
> end up with high-molecular weight concatomers? I'm getting some
> high-weight stuff when I run a gel and the transformations aren't working.
Thanks to everyone who has helped me out with this. It appears my
problem was too much DNA in the reaction -- I dropped the concentration
from ~1 ug to 80 ng and it worked wonderfully.
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