Sandra Pena De Ortiz
spenadeo at lonestar.jpl.utsa.edu
Sat Jul 6 21:41:51 EST 1996
Hi I made a small addition to my response that I hope will clarify it.
On Thu, 4 Jul 1996, Sandra Pena De Ortiz wrote:
> On Fri, 21 Jun 1996, MARK PEGGIE wrote:
> > Hi, I wonder if someone could give me some advice. I am about to make a
> > subtractive cDNA library but am not sure of the best approach. I was going to
> > follow a biotin/streptavidin method but found the PCR-Select cDNA Subtraction
> > kit from Clontech. Has anyone tried this kit - what are the advantages and
> > disadvantages.
> > I'm sorry if this has been covered already.
> > Cheers
> > Mark Peggie
> We have been constructing subtracted cDNA libraries for some time and
> have used sevearl approaches. The best approaches have been those using
> the biotin/streptavidin steps for subtraction. One student in our lab
> who had succesfully constructed several
> subtracted libraries
(with cloning strategies established in our lab, but when he )
tried out the Clontech kit and it did not work. In
> my opinion, if you carefully design a good cloning and subtraction strategy
> before starting you experiment you have no need of using the kit and
> will get results in a reasonable amount of time.
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