Subtractive cDNA

Sandra Pena De Ortiz spenadeo at lonestar.jpl.utsa.edu
Sat Jul 6 21:41:51 EST 1996



Hi I made a small addition to my response that I hope will clarify it.

 

On Thu, 4 Jul 1996, Sandra Pena De Ortiz wrote:

> 
> 
> On Fri, 21 Jun 1996, MARK PEGGIE wrote:
> 
> > Hi,  I wonder if someone could give me some advice.  I am about to make a 
> > subtractive cDNA library but am not sure of the best approach.  I was going to 
> > follow a biotin/streptavidin method but found the PCR-Select cDNA Subtraction 
> > kit from Clontech.  Has anyone tried this kit - what are the advantages and 
> > disadvantages.
> > I'm sorry if this has been covered already.
> > 
> > Cheers
> > 
> > Mark Peggie
> > 
> > 
> > 
> -------
> 
> Hi:
> 
> We have been constructing subtracted cDNA libraries for some time and 
> have used sevearl approaches.  The best approaches have been those using 
> the biotin/streptavidin steps for subtraction.  One student in our lab 
> who had succesfully constructed several 
>  subtracted libraries


(with cloning strategies established in our lab, but when he )


 tried out the Clontech kit and it did not work.  In 
> my opinion, if you carefully design a good cloning and subtraction strategy 
> before starting you experiment  you have no need of using the kit and 
> will get results in a reasonable amount of time.
> 
> SPO
> 
> 
> 



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