Rapid hybridization buffer

Nick Jacobsen jacobsen at cf.ac.uk
Mon Jul 8 06:12:19 EST 1996


Hi,
  I use rapid hyb buffer and find it to be very good. All I do is prehyb 
for about 1 hour with 10mls buffer and add the probe straight to the 
buffer. I wouldnt dilute it since the glupyness of the buffer is 
essential for efficient hybridisation. It says you can do hybridisations 
in a few hours but I think this would occur for a perfect blot and probe.

cheers 

Nick. 




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