temperature induction in BACTERIA

marcvdc at lmb1.rug.ac.be marcvdc at lmb1.rug.ac.be
Sun Jul 7 20:02:01 EST 1996


Dear Netters,

In smale scale (4ml) temperature induction (28~C to 42~C) we get nice 
production of recombinant protein in bacteria.  However, if we switch to large 
1 liter culture, we have much less production of the recombinant protein. This 
temperature shift was done by diluting the culture 1/2 with 56~C medium and 
keeping the culture at 42~C.

Does someone know how to do excellent temperatur induction on 1 liter bacteria 
culture.


Thank you in advance,

Marc



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