temperature induction of BACTERIA

marcvdc at lmb1.rug.ac.be marcvdc at lmb1.rug.ac.be
Sun Jul 7 19:55:49 EST 1996


Dear Netter,


On smale scale (4 ml) temperature induction (28~C to 42~C) of recombinant 
protein in bacteria we have nice expression of the recombinant 
protein. However if we switch to 1 liter culture, the temperature induction 
does not yield the expected amount of recombinant protein.  We did this 
temperature shift by diluting the culture 1/2 with medium of 56`C and keeping 
the culture at 42~C.

Does someone have a nice idea for  good temperature induction on large scale 
bacteria culture?


Thank you very much for your respons,


Marc  



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