4 way ligation avoided !!!!!!

Camella Bailey cbailey at umabnet.ab.umd.edu
Mon Jul 8 13:18:30 EST 1996


You could also try designing your pcr primers with NcoI and HindIII half 
sites at their respective ends, thereby eliminating the need to digest the 
pcr product before ligation.

Camella Bailey
CVD, UMAB



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