ECL direct with oligos

[Juergen Sasse (Path)]

I am trying to hybridize different oligos to Southerns of PFG separated 
chromosomes. I have tryed radioactive labeling and the ECL indirect oligo 
tailing kit with varying degrees of success. Sometimes I get good 
hybridizations and sometimes a lot of background. I haven't detected a 
pattern in it yet. I would like to try to use the ECL direct kit after 
random elongation of the oligos by Terminal Transferase and see how that 
works. There is a paper in Cell 70:1059-1068 which describes the use of a 
51mer with the ECL direct kit after random TT elongation. I was wondering 
if somebody has experience with 20-30mers as substrates for the ECL 
direct kit after elongation with TT. Does it still work with short 
primers i.e. specificity of the hybridization? Does somebody have a 
detailed protocol? The Cell paper isn't very forthcoming on that.
Thanx a lot, Juergen.
(please send email to js1 at mole.bio.cam.ac.uk)