DNA ligation

[u.381 at inserm.u-strasbg.fr]

dear net people

I have some problems for subcloning DNA fragments (0.2 - 6 kb) eluted 
from agarose gels. I have already used several methods and kits without 
optimal results (NaI solubilization, low melting point agarose, agarose 
free of ligase inhibitors, freezing method, etc).
Has anybody an answer for this problem.
Thanks in advance

Jean-Noel Freund, INSERM U381 Strasbourg France
u.381 at inserm.u-strasbg.fr