T cell receptor PCR

Silvia Spitzer SSPITZER at path.som.sunysb.edu
Tue Jul 9 12:36:49 EST 1996


Is someone using PCR to study T cell receptor rearrangements?
I am following the McCarthy paper, setting up 4 reactions per patient VJ1,
VJ2, D1J2, D2J2 but I do not get any specific band, lots of background
(higher than 300bp).
Does anyone have any suggestions, protocols, conditions, etc.?
Thanks,
Silvia Spitzer
SUNY Stony Brook
SSPITZER at path.som.sunysb.edu



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