T cell receptor PCR

Silvia Spitzer SSPITZER at path.som.sunysb.edu
Tue Jul 9 12:36:49 EST 1996

Is someone using PCR to study T cell receptor rearrangements?
I am following the McCarthy paper, setting up 4 reactions per patient VJ1,
VJ2, D1J2, D2J2 but I do not get any specific band, lots of background
(higher than 300bp).
Does anyone have any suggestions, protocols, conditions, etc.?
Silvia Spitzer
SUNY Stony Brook
SSPITZER at path.som.sunysb.edu

More information about the Methods mailing list