adaptors: 5' blunt-end phosphorilated?

orlando dominguez IKHT5 at cc.uab.es
Wed Jul 10 02:49:45 EST 1996


Does anyone knows whether..
..ligation efficiency of an adaptor to a phosphorylated insert at blunt
ends is worse when the adaptor is 5' blunt-end non-phosphorylated?

A different thing would be the stability of the short oligomer (just
duplexed to the long one) on downstream steps. Could be lost?

Thanks,

Orlando




More information about the Methods mailing list