adaptors: 5' blunt-end phosphorilated?

orlando dominguez IKHT5 at
Wed Jul 10 02:49:45 EST 1996

Does anyone knows whether..
..ligation efficiency of an adaptor to a phosphorylated insert at blunt
ends is worse when the adaptor is 5' blunt-end non-phosphorylated?

A different thing would be the stability of the short oligomer (just
duplexed to the long one) on downstream steps. Could be lost?



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