anti-6Xhis antibody - any new info?
Mark.Prescott at med.monash.edu.au
Thu Jul 11 01:06:25 EST 1996
mcc9 at cornell.edu (Maria C. Costanzo) wrote:
>I would like to find out whether an antibody is available that would
>recognize a his-tag (only) and would give a strong signal without a high
>background against total yeast proteins. Yes, I did scan the archives for
>this newsgroup and it seems that as of a few months ago, there were 3
>sources for anti-his tag antibodies. One (from Qiagen) seemed to require
>3 specific residues next to the his tag for recognition; no one had much
>experience with the other two. Anything new on this subject?
A monoclonal antibody against hexahistidine (alone) is available from
Dianova Gmbh, Postfach 30 1250, 20305 Hamburg (FAX 040 3221 90). We have
used it successfully for the detection of yeast mitochondrial proteins
tagged at their C-terminus with hexahistidine. Our work with using this
antisera is scheduled to appear in Analytical Biochemistry vol. 238, No.
1, 1996 pp.14-18.
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