gunk in RNA extraction

GERALDINE KAMINSKI gzk at ollamh.ucd.ie
Wed Jul 10 07:23:31 EST 1996


We usually extract viral RNA from serum using Ultraspec (a guanidinium 
method). The aqueous layer is removed and an equal volume of propanol added with 
1ug glycogen to precipitate out the RNA.  The tube is then centrifuged and
the pellet washed with 1ml 75%ethanol.  My collegue added isopropanol in
error at this point and got a large smear of what looked like salts down
the side og the tube.  Anyone any idea what this is as it looks similar to stuff
that we think inhibits our RT reaction?
Thanks Geraldine.



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