electroporation of cosmids
egreif at cc.umanitoba.ca
Thu Jul 11 11:19:47 EST 1996
We electroporate E. coli all the time with normal sized vectors using the
standard conditions i.e. 1.25-1.8 kV with 0.1 cm cuvettes. We are now
thinking of testing a cosmid ligation (cosmid is 4.5 kb, inserts are 35-45
kb) by direct electroporation into E. coli as we don't want to waste
anymore packaging extracts until we know we are gettting a fair number of
recombinant cosmids from the ligation.
Question - Should we use different electroporation conditions for such
large plasmids? Different voltage? Increase or decrease pulse time?
Hey, hey, hey, hey! It was the DNA.
Hey, hey, hey, hey! That made me this way.
More information about the Methods