Ficoll removal from organelle lysates

Dr E. Buxbaum EB15 at le.ac.uk
Thu Jul 11 09:03:47 EST 1996


If you don't need active proteins, you can use Chloroform/Methanol 
precipitation (published by Wessel & Fluegge in Anal. Biochem, a couple 
of years ago). I find that 8 M Urea, 1% SDS, 50 mM Tris-HCl pH 10 is good 
for solubilisation of these precipitates. 




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