Help for electroporation of E.coli starved cells

Bregeon Damien bregeon at ijm.jussieu.fr
Thu Jul 11 06:05:27 EST 1996


 I'm trying to electroporate E.coli starved cells in order to study the mismatch
repair system. For this study, I need a high efficiency  of transformation
(more than 10e8 transformants per ug DNA per mL) and my problem is that my
cells seem to not be enough competent. So I'm looking for a method which
increases the transformation efficiency by electroporation.



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