Help for electroporation of E.coli starved cells

Taddei Francois taddei at moka.ccr.jussieu.fr
Thu Jul 11 04:43:57 EST 1996


 I'm trying to electroporate E.coli starved cells in order to study the mismatch
repair system. For this study, I need a high efficiency  of transformation
(more than 10e8 transformants per ug DNA per mL) and my problem is that my
cells seem to not be enough competent. So I'm looking for a method which
increases the transformation efficiency by electroporation.

-- 
Francois Taddei
Laboratoire de Mutagenese, 
Institut J. Monod, 
Universite Paris 7
2, place Jussieu, 75251, Paris, France

Tel:  33 (1) 44 27 47 31
Fax:  33 (1) 44 27 57 16         
E-mail taddei at ccr.jussieu.fr



More information about the Methods mailing list