4 way ligation avoided !!!!!!

Gabi Gerlitz gabiger at zoot.tau.ac.il
Thu Jul 11 23:24:16 EST 1996


Camella Bailey wrote:
> 
> You could also try designing your pcr primers with NcoI and HindIII half
> sites at their respective ends, thereby eliminating the need to digest the
> pcr product before ligation.
> 
> Camella Bailey
> CVD, UMAB


Hi,

The enzymes don't cut single strand.

Gabi.



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