unbiotinylating streptavidin ... ?

LeeSylvers leesylvers at aol.com
Fri Jul 12 18:10:40 EST 1996


I have tried the monomeric avidin before for binding of nucleic acids and
subsequent release, but was dissatisfied for two reasons: 1) the
pretreatment to insure that avidin was monomeric was tedious; and, 2) I
had very poor binding efficiency of my biotinylated nucleic acid.  I will
admit, however, that the material that did bind was easily removed by
competition with free biotin.



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