unbiotinylating streptavidin ... ?
leesylvers at aol.com
Fri Jul 12 18:10:40 EST 1996
I have tried the monomeric avidin before for binding of nucleic acids and
subsequent release, but was dissatisfied for two reasons: 1) the
pretreatment to insure that avidin was monomeric was tedious; and, 2) I
had very poor binding efficiency of my biotinylated nucleic acid. I will
admit, however, that the material that did bind was easily removed by
competition with free biotin.
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