unbiotinylating streptavidin ... ?

LeeSylvers leesylvers at aol.com
Fri Jul 12 18:10:40 EST 1996

I have tried the monomeric avidin before for binding of nucleic acids and
subsequent release, but was dissatisfied for two reasons: 1) the
pretreatment to insure that avidin was monomeric was tedious; and, 2) I
had very poor binding efficiency of my biotinylated nucleic acid.  I will
admit, however, that the material that did bind was easily removed by
competition with free biotin.

More information about the Methods mailing list