Poly C PCR, anyone?
brett_beitzel at MSMTP.FS.SACI.ORG
Fri Jul 12 14:41:31 EST 1996
Subject:Poly C PCR, anyone? Date: 7/12/96
Has anyone ever used a poly C primer for PCR (or another homopolynucleotide
primer?) I am trying to polyG tail a piece of DNA with Terminal transferase
and then PCR this tailed fragment with oligo(dC)16. I know that my TdT is
working, but I can't get any product in my PCR. I am wondering if there is
something odd that happens when you try to use a homopolynucleotide primer.
Also, any secrets for using oligo(dT) primers? I've tried them a couple of
times, but have had a lot of difficulty getting a specific product.
Thanks for any info,
brett_beitzel at msmtp.idde.saci.org
Cancer Therapy and Research Center
San Antonio, TX
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