unbiotinylating streptavidin ... ?

Dima Klenchin klenchin at facstaff.wisc.edu
Fri Jul 12 10:25:58 EST 1996

In article <31E6618A.7B10 at uic.edu>, Keld Sorensen <KeldS at uic.edu> wrote:
#wo ways to "elute" biotinylated "stuff" off avidin:
#use "momomeric avidin" supports - these have much
#lower affinity and you can actually elute with a biotin

I second this. Just add a lot of biotin and wait a lot (o/n).

- Dima

More information about the Methods mailing list