In article <31E6618A.7B10 at uic.edu>, Keld Sorensen <KeldS at uic.edu> wrote: #wo ways to "elute" biotinylated "stuff" off avidin: # #use "momomeric avidin" supports - these have much #lower affinity and you can actually elute with a biotin #solution. I second this. Just add a lot of biotin and wait a lot (o/n). - Dima