Blue color comes late lately in yeast 2 hybrid
rafael at howard.med.utah.edu
Sat Jul 13 19:14:11 EST 1996
On 11 Jul 1996, Riekeltje Koedood wrote:
> First I should mention that we do X-Gal/agarose overlays to see our
> blue colonies.
So, are you breaking the cells in any way? Using the filter lift colony
assay, cells are broken in liquid nitrogen before adding the X-gal
solution. How do you break your cells? How long doe it takes in developing
the blue color using the agar overlay?
I am really interested in knowing how to advoid the somewhat cumbersome
method of the liquid nitrogen. But it looks like that your system does
not work properly... any comments?
Rafael Maldonado | 'No te creas todo
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