Ni chelate affinity chromatography

Mr. Chris Hoyle bmbckh at
Tue Jul 16 03:47:07 EST 1996

In article <4s6jim$7p1 at>
ez005679 at ([user unknown]) writes:

> I have a problem of purifying over expressed protein using His-binding 
> resins provided by Novagene company.  The problem is my protein does not 
> bind to the column, even after I tried urea denaturation.   The protein I 
> am trying to purify is a memebrane binding protein and was shown to be 
> present in the binding  and washing solutions after loading, but 
> not in the eluting solution.    Could anybody please give me some advice 
> on this column purification?   Thanks in advance
> Yusen 

Could you mail your conditions? We use the above method purification
membrane proteins.

Chris Hoyle

   Department of Biochemistry and Molecular Biology
University of Leeds             phone +44 0113 2333172
Leeds LS2 9JT                   FAX   +44 0113 2333167
Great Britain           e-mail BMBCKH at

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