Tm calculation short and long oligo's

Ke Wei victorke at acpub.duke.edu
Tue Jul 16 10:16:17 EST 1996


On Wed, 10 Jul 1996, kevin gilbride wrote:

> Date: Wed, 10 JUL 1996 10:28:49 -0400 
> From: kevin gilbride <gilbride at bu.edu>
> Newgroups: bionet.molbio.methds-reagnts
> Subject: Re: Tm calculation short and long oligo's 
> 
> In article <4r5mic$l6b at news.xs4all.nl>, marker at xs4all.nl (Niels Groot) wrote:
> 
> > Hello
> > 
> > I'm looking for the formula's to calculate Tm for oligo's 
> 
> 
> 
> 
> If memory serves me correctlty take the number of c/g's and multiply by
> 7.5 this will give Tm.
> 
> good luck,
> kevin
> 
> 

I use another formula for my PCR needs: Tm = # of c/g x 4 + # of a/t x 2.
It works for me all these years.






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