Tm calculation short and long oligo's
victorke at acpub.duke.edu
Tue Jul 16 10:16:17 EST 1996
On Wed, 10 Jul 1996, kevin gilbride wrote:
> Date: Wed, 10 JUL 1996 10:28:49 -0400
> From: kevin gilbride <gilbride at bu.edu>
> Newgroups: bionet.molbio.methds-reagnts
> Subject: Re: Tm calculation short and long oligo's
> In article <4r5mic$l6b at news.xs4all.nl>, marker at xs4all.nl (Niels Groot) wrote:
> > Hello
> > I'm looking for the formula's to calculate Tm for oligo's
> If memory serves me correctlty take the number of c/g's and multiply by
> 7.5 this will give Tm.
> good luck,
I use another formula for my PCR needs: Tm = # of c/g x 4 + # of a/t x 2.
It works for me all these years.
More information about the Methods